Identification and characterization of Ixodes scapularis antigens that elicit tick immunity using yeast surface display

Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary a...

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Bibliographic Details
Published in:	PloS one 2011-01, Vol.6 (1), p.e15926-e15926
Main Authors:	Schuijt, Tim J, Narasimhan, Sukanya, Daffre, Sirlei, DePonte, Kathleen, Hovius, Joppe W R, Van't Veer, Cornelis, van der Poll, Tom, Bakhtiari, Kamran, Meijers, Joost C M, Boder, Eric T, van Dam, Alje P, Fikrig, Erol
Format:	Article
Language:	eng
Subjects:	Acari Animals Antibodies Antigens Antigens - blood Antigens - immunology Antigens - isolation & purification Arachnids Baking yeast Biology Bites Bites (Injuries) Bites and stings Borrelia Breast cancer Cell cycle Coagulants Drosophila Feeding Identification Immune serum Immunity Immunization Immunization - methods Immunoglobulins Insects Internal medicine Ixodes - immunology Ixodes ricinus Ixodidae Libraries Medicine Neurogenesis Nymph Pathogens Peptide Library Proteins Rabbits Saccharomyces cerevisiae Salivary gland Salivary Glands - immunology Sexes Ticks Ticks - immunology Vectors (Biology) Viral antibodies Yeasts Zoonoses
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Summary:	Repeated exposure of rabbits and other animals to ticks results in acquired resistance or immunity to subsequent tick bites and is partially elicited by antibodies directed against tick antigens. In this study we demonstrate the utility of a yeast surface display approach to identify tick salivary antigens that react with tick-immune serum. We constructed an Ixodes scapularis nymphal salivary gland yeast surface display library and screened the library with nymph-immune rabbit sera and identified five salivary antigens. Four of these proteins, designated P8, P19, P23 and P32, had a predicted signal sequence. We generated recombinant (r) P8, P19 and P23 in a Drosophila expression system for functional and immunization studies. rP8 showed anti-complement activity and rP23 demonstrated anti-coagulant activity. Ixodes scapularis feeding was significantly impaired when nymphs were fed on rabbits immunized with a cocktail of rP8, rP19 and rP23, a hall mark of tick-immunity. These studies also suggest that these antigens may serve as potential vaccine candidates to thwart tick feeding.
ISSN:	1932-6203 1932-6203