RT-PCR Assays for Seven Serotypes of Epizootic Haemorrhagic Disease Virus & Their Use to Type Strains from the Mediterranean Region and North America

Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assay...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2010-09, Vol.5 (9), p.e12782
Main Authors: Maan, Narender S, Maan, Sushila, Nomikou, Kyriaki, Johnson, Donna J, El Harrak, Mehdi, Madani, Hafsa, Yadin, Hagai, Incoglu, Serife, Yesilbag, Kadir, Allison, Andrew B, Stallknecht, David E, Batten, Carrie, Anthony, Simon J, Mertens, Peter P. C, Kremer, Eric J
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibodies
Assaying
Bluetongue
Bovidae
Capsid protein
Capsid Proteins - genetics
Cattle
cattle diseases
Cattle Diseases - virology
Cell Line
Cricetinae
Diagnostic systems
Disease
DNA primers
Epizootic hemorrhagic disease virus
Genes
Genetic diversity
Genomes
Genomics
Health aspects
Hemorrhagic disease
Hemorrhagic Disease Virus, Epizootic - classification
Hemorrhagic Disease Virus, Epizootic - genetics
Hemorrhagic Disease Virus, Epizootic - isolation & purification
Infections
Infectious Diseases/Viral Infections
Laboratories
Mediterranean Region
Molecular Sequence Data
Morbidity
mortality
neutralization tests
neutralizing antibodies
North America
Odocoileus
Oligonucleotides
Phylogenetics
Phylogeny
Polymerase chain reaction
Primers
Proteins
Public Health and Epidemiology/Infectious Diseases
Reoviridae Infections - veterinary
Reoviridae Infections - virology
reverse transcriptase polymerase chain reaction
Reverse Transcriptase Polymerase Chain Reaction - methods
Ruminantia
ruminants
Serotypes
Vectors (Biology)
Veterinary colleges
Veterinary medicine
Viral proteins
Virology
Virology/Diagnosis
Virology/Emerging Viral Diseases
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus “serotype”, as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV “types”. Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0012782