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Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes
Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses,...
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Published in: | PloS one 2010-02, Vol.5 (2), p.e9097-e9097 |
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description | Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA. |
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However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0009097</identifier><identifier>PMID: 20140098</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino Acid Sequence ; Analysis ; Animals ; Antibodies ; Antibodies, Viral - blood ; Antigenic determinants ; Antigens ; Antigens, Viral - immunology ; Antisera ; Avian flu ; Avian influenza ; Avian influenza viruses ; Baculovirus ; Biochemistry/Drug Discovery ; Birds ; Blotting, Western ; Diagnostic software ; Diagnostic systems ; Enzyme-Linked Immunosorbent Assay ; Epitope Mapping ; Epitopes ; Epitopes - genetics ; Epitopes - immunology ; Epitopes - metabolism ; Exo-a-sialidase ; Genetic aspects ; Genetics and Genomics/Genetics of Disease ; Hemagglutinin Glycoproteins, Influenza Virus - genetics ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Hemagglutinin Glycoproteins, Influenza Virus - metabolism ; Hemagglutinins ; Immune Sera - immunology ; Influenza ; Influenza A virus - classification ; Influenza A virus - genetics ; Influenza A virus - immunology ; Influenza vaccines ; Lectins ; Molecular Sequence Data ; Mutation ; Orthomyxoviridae ; Pandemics ; Pathogenicity ; Pathogens ; Peptide libraries ; Peptides ; Proteins ; Public Health and Epidemiology/Immunization ; Public Health and Epidemiology/Infectious Diseases ; Rabbits ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Sequence Homology, Amino Acid ; Vaccines ; Virology/Diagnosis ; Virology/Vaccines ; Viruses</subject><ispartof>PloS one, 2010-02, Vol.5 (2), p.e9097-e9097</ispartof><rights>COPYRIGHT 2010 Public Library of Science</rights><rights>2010 Mueller et al. 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Performed the experiments: MM SR RB NR. Analyzed the data: MM. Wrote the paper: MM MAH.</notes><abstract>Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20140098</pmid><doi>10.1371/journal.pone.0009097</doi><tpages>e9097</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acid Sequence Analysis Animals Antibodies Antibodies, Viral - blood Antigenic determinants Antigens Antigens, Viral - immunology Antisera Avian flu Avian influenza Avian influenza viruses Baculovirus Biochemistry/Drug Discovery Birds Blotting, Western Diagnostic software Diagnostic systems Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes Epitopes - genetics Epitopes - immunology Epitopes - metabolism Exo-a-sialidase Genetic aspects Genetics and Genomics/Genetics of Disease Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology Hemagglutinin Glycoproteins, Influenza Virus - metabolism Hemagglutinins Immune Sera - immunology Influenza Influenza A virus - classification Influenza A virus - genetics Influenza A virus - immunology Influenza vaccines Lectins Molecular Sequence Data Mutation Orthomyxoviridae Pandemics Pathogenicity Pathogens Peptide libraries Peptides Proteins Public Health and Epidemiology/Immunization Public Health and Epidemiology/Infectious Diseases Rabbits Recombinant Proteins - immunology Recombinant Proteins - metabolism Sequence Homology, Amino Acid Vaccines Virology/Diagnosis Virology/Vaccines Viruses |
title | Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes |
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