Antigenic characterization of recombinant hemagglutinin proteins derived from different avian influenza virus subtypes

Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses,...

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Bibliographic Details
Published in:PloS one 2010-02, Vol.5 (2), p.e9097-e9097
Main Authors: Mueller, Matthias, Renzullo, Sandra, Brooks, Roxann, Ruggli, Nicolas, Hofmann, Martin A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis
Animals
Antibodies
Antibodies, Viral - blood
Antigenic determinants
Antigens
Antigens, Viral - immunology
Antisera
Avian flu
Avian influenza
Avian influenza viruses
Baculovirus
Biochemistry/Drug Discovery
Birds
Blotting, Western
Diagnostic software
Diagnostic systems
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Epitopes
Epitopes - genetics
Epitopes - immunology
Epitopes - metabolism
Exo-a-sialidase
Genetic aspects
Genetics and Genomics/Genetics of Disease
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Hemagglutinin Glycoproteins, Influenza Virus - metabolism
Hemagglutinins
Immune Sera - immunology
Influenza
Influenza A virus - classification
Influenza A virus - genetics
Influenza A virus - immunology
Influenza vaccines
Lectins
Molecular Sequence Data
Mutation
Orthomyxoviridae
Pandemics
Pathogenicity
Pathogens
Peptide libraries
Peptides
Proteins
Public Health and Epidemiology/Immunization
Public Health and Epidemiology/Infectious Diseases
Rabbits
Recombinant Proteins - immunology
Recombinant Proteins - metabolism
Sequence Homology, Amino Acid
Vaccines
Virology/Diagnosis
Virology/Vaccines
Viruses
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Description
Summary:Since the advent of highly pathogenic variants of avian influenza virus (HPAIV), the main focus of avian influenza research has been the characterization and detection of HPAIV hemagglutinin (HA) from H5 and H7 subtypes. However, due to the high mutation and reassortation rate of influenza viruses, in theory any influenza strain may acquire increased pathogenicity irrespective of its subtype. A comprehensive antigenic characterization of influenza viruses encompassing all 16 HA and 9 neuraminidase subtypes will provide information useful for the design of differential diagnostic tools, and possibly, vaccines. We have expressed recombinant HA proteins from 3 different influenza virus HA subtypes in the baculovirus system. These proteins were used to generate polyclonal rabbit antisera, which were subsequently employed in epitope scanning analysis using peptide libraries spanning the entire HA. Here, we report the identification and characterization of linear, HA subtype-specific as well as inter subtype-conserved epitopes along the HA proteins. Selected subtype-specific epitopes were shown to be suitable for the differentiation of anti-HA antibodies in an ELISA.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0009097