Interaction of Saccharomyces boulardii with Salmonella enterica serovar Typhimurium protects mice and modifies T84 cell response to the infection

Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-kappaB. Wo...

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Bibliographic Details
Published in:PloS one 2010-01, Vol.5 (1), p.e8925-e8925
Main Authors: Martins, Flaviano S, Dalmasso, Guillaume, Arantes, Rosa M E, Doye, Anne, Lemichez, Emmanuel, Lagadec, Patricia, Imbert, Veronique, Peyron, Jean-François, Rampal, Patrick, Nicoli, Jacques R, Czerucka, Dorota
Format: Article
Language:English
Subjects:
Activation
Animals
Bacteria
Bacterial Adhesion
Baking yeast
Binding proteins
Cancer
Cell Biology/Cell Signaling
Cellular signal transduction
Cholera
Colorectal cancer
Colorectal carcinoma
Confocal microscopy
Diarrhea
E coli
Escherichia coli
Freeze Drying
Functional foods
Functional foods & nutraceuticals
Gastroenterology and Hepatology/Gastrointestinal Infections
Gene expression
GTP-binding protein
Health aspects
Health promotion
Immune system
Infections
Infectious Diseases/Bacterial Infections
Infectious Diseases/Gastrointestinal Infections
Inflammation
Inflammatory response
Interleukin 8
Invasiveness
JNK protein
Kinases
Laboratory animals
Life Sciences
Liver
Liver - microbiology
Mice
Microbiology/Applied Microbiology
Microbiology/Medical Microbiology
Microscopy
Microscopy, Electron, Transmission
Mitogen-Activated Protein Kinases - metabolism
NF-kappa B - metabolism
NF-κB protein
Nuclear transport
Pathogenesis
Permeability
Phagocytosis
Physical fitness
Prevention
Probiotics
Protein binding
Proteins
Rac1 protein
Saccharomyces
Saccharomyces - physiology
Saccharomyces cerevisiae
Salmonella
Salmonella enterica - pathogenicity
Salmonella Infections - immunology
Salmonella Infections - mortality
Salmonella Infections - prevention & control
Salmonella Typhimurium
Shigella
Signal Transduction
Stem cells
Translocation
Tropical diseases
Yeast
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Summary:Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-kappaB. Worldwide interest in the use of functional foods containing probiotic bacteria for health promotion and disease prevention has increased significantly. Saccharomyces boulardii is a non-pathogenic yeast used as a probiotic in infectious diarrhea. In this study, we reported that S. boulardii (Sb) protected mice from Salmonella enterica serovar Typhimurium (ST)-induced death and prevented bacterial translocation to the liver. At a molecular level, using T84 human colorectal cancer cells, we demonstrate that incubation with Sb before infection totally abolished Salmonella invasion. This correlates with a decrease of activation of Rac1. Sb preserved T84 barrier function and decreased ST-induced IL-8 synthesis. This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2, p38 and JNK as well as on activation of NF-kappaB. Electron and confocal microscopy experiments showed an adhesion of bacteria to yeast cells, which could represent one of the mechanisms by which Sb exerts its protective effects. Sb shows modulating effects on permeability, inflammation, and signal transduction pathway in T84 cells infected by ST and an in vivo protective effect against ST infection. The present results also demonstrate that Sb modifies invasive properties of Salmonella.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0008925