Sequence-based analysis uncovers an abundance of non-coding RNA in the total transcriptome of Mycobacterium tuberculosis

RNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence r...

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Bibliographic Details
Published in:PLoS pathogens 2011-11, Vol.7 (11), p.e1002342-e1002342
Main Authors: Arnvig, Kristine B, Comas, Iñaki, Thomson, Nicholas R, Houghton, Joanna, Boshoff, Helena I, Croucher, Nicholas J, Rose, Graham, Perkins, Timothy T, Parkhill, Julian, Dougan, Gordon, Young, Douglas B
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biology
Gene expression
Gene Expression Regulation, Bacterial
Genetic aspects
Genomics
Mice
Mice, Inbred C57BL
Microbiology
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - pathogenicity
Noncoding DNA
Oligonucleotide Array Sequence Analysis
Physiological aspects
Proteins
Ribosomal RNA
Risk factors
RNA sequencing
RNA, Bacterial - analysis
RNA, Bacterial - genetics
RNA, Untranslated - analysis
RNA, Untranslated - genetics
Sequence Analysis, RNA
Transcriptome
Tuberculosis
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Summary:RNA sequencing provides a new perspective on the genome of Mycobacterium tuberculosis by revealing an extensive presence of non-coding RNA, including long 5' and 3' untranslated regions, antisense transcripts, and intergenic small RNA (sRNA) molecules. More than a quarter of all sequence reads mapping outside of ribosomal RNA genes represent non-coding RNA, and the density of reads mapping to intergenic regions was more than two-fold higher than that mapping to annotated coding sequences. Selected sRNAs were found at increased abundance in stationary phase cultures and accumulated to remarkably high levels in the lungs of chronically infected mice, indicating a potential contribution to pathogenesis. The ability of tubercle bacilli to adapt to changing environments within the host is critical to their ability to cause disease and to persist during drug treatment; it is likely that novel post-transcriptional regulatory networks will play an important role in these adaptive responses.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1002342