Protection from the 2009 H1N1 pandemic influenza by an antibody from combinatorial survivor-based libraries

Influenza viruses elude immune responses and antiviral chemotherapeutics through genetic drift and reassortment. As a result, the development of new strategies that attack a highly conserved viral function to prevent and/or treat influenza infection is being pursued. Such novel broadly acting antivi...

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Bibliographic Details
Published in:PLoS pathogens 2010-07, Vol.6 (7), p.e1000990-e1000990
Main Authors: Kashyap, Arun K, Steel, John, Rubrum, Adam, Estelles, Angeles, Briante, Raffaella, Ilyushina, Natalia A, Xu, Li, Swale, Ryann E, Faynboym, Aleksandr M, Foreman, Pamela K, Horowitz, Michael, Horowitz, Lawrence, Webby, Richard, Palese, Peter, Lerner, Richard A, Bhatt, Ramesh R
Format: Article
Language:English
Subjects:
Amino acids
Animal models
Animals
Antibodies
Antibodies, Monoclonal - therapeutic use
Antibodies, Viral - therapeutic use
Avian flu
Biochemistry/Drug Discovery
Biotechnology
Cell culture
Combinatorial libraries
Cross Reactions - immunology
Disease Models, Animal
Disease Outbreaks
DNA sequencing
Epidemics
Experiments
Genetic aspects
Genetic drift
Humans
Immune response
Immunity
Immunology/Antigen Processing and Recognition
Immunology/Immune Response
Immunology/Immunity to Infections
Immunotherapy - methods
Infection
Infections
Infectious Diseases
Infectious Diseases/Respiratory Infections
Infectious Diseases/Viral Infections
Influenza
Influenza A Virus, H1N1 Subtype - immunology
Influenza A Virus, H5N1 Subtype - immunology
Influenza virus
Influenza, Human - drug therapy
Influenza, Human - prevention & control
Libraries
Methods
Mice
Monoclonal antibodies
Nucleotide sequencing
Orthomyxoviridae Infections - drug therapy
Orthomyxoviridae Infections - prevention & control
Pandemics
Pathogens
Prevention
Properties
Risk factors
Seasonal variations
Sulfur dioxide
Survivors
Swine flu
Swine influenza
United States
Vaccines
Viral antibodies
Viruses
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Summary:Influenza viruses elude immune responses and antiviral chemotherapeutics through genetic drift and reassortment. As a result, the development of new strategies that attack a highly conserved viral function to prevent and/or treat influenza infection is being pursued. Such novel broadly acting antiviral therapies would be less susceptible to virus escape and provide a long lasting solution to the evolving virus challenge. Here we report the in vitro and in vivo activity of a human monoclonal antibody (A06) against two isolates of the 2009 H1N1 pandemic influenza virus. This antibody, which was obtained from a combinatorial library derived from a survivor of highly pathogenic H5N1 infection, neutralizes H5N1, seasonal H1N1 and 2009 "Swine" H1N1 pandemic influenza in vitro with similar potency and is capable of preventing and treating 2009 H1N1 influenza infection in murine models of disease. These results demonstrate broad activity of the A06 antibody and its utility as an anti-influenza treatment option, even against newly evolved influenza strains to which there is limited immunity in the general population.
ISSN:1553-7374
1553-7366
1553-7374
DOI:10.1371/journal.ppat.1000990