Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries

Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in a few countries. Two hyperendemic areas were chosen: Huacullani, Northern Altiplano, Bolivia, representing the Altiplanic transmission pattern with high prevalences and intensities; Cajamarca valley, P...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2012-09, Vol.6 (9), p.e1812-e1812
Main Authors: Valero, María Adela, Periago, María Victoria, Pérez-Crespo, Ignacio, Angles, René, Villegas, Fidel, Aguirre, Carlos, Strauss, Wilma, Espinoza, José R, Herrera, Patricia, Terashima, Angelica, Tamayo, Hugo, Engels, Dirk, Gabrielli, Albis Francesco, Mas-Coma, Santiago
Format: Article
Language:English
Subjects:
Adolescent
Antigens
Antigens, Helminth - analysis
Bolivia
Causes of
Child
Child, Preschool
Clinical Laboratory Techniques - methods
Demographic aspects
Diagnosis
Enzyme-Linked Immunosorbent Assay - methods
Fascioliasis - diagnosis
Feces - chemistry
Feces - parasitology
Female
Fluke infections
Humans
Male
Mass Screening - methods
Medicine
Parasitology - methods
Peru
Sensitivity and Specificity
Specimen Handling - methods
Testing
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Summary:Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in a few countries. Two hyperendemic areas were chosen: Huacullani, Northern Altiplano, Bolivia, representing the Altiplanic transmission pattern with high prevalences and intensities; Cajamarca valley, Peru, representing the valley pattern with high prevalences but low intensities. Coprological sample collection, transport and study procedures were analyzed to improve individual diagnosis and subsequent treatments and surveillance activities. Therefore, a coproantigen-detection technique (MM3-COPRO ELISA) was evaluated, using classical techniques for egg detection for comparison. A total of 436 and 362 stool samples from schoolchildren of Huacullani and Cajamarca, respectively, were used. Positive samples from Huacullani were 24.77% using the MM3-COPRO technique, and 21.56% using Kato-Katz. Positive samples from Cajamarca were 11.05% using MM3-COPRO, and 5.24% using rapid sedimentation and Kato-Katz. In Huacullani, using Kato-Katz as gold standard, sensitivity and specificity were 94.68% and 98.48%, respectively, and using Kato-Katz and COPRO-ELISA test together, they were 95.68% and 100%. In Cajamarca, using rapid sedimentation and Kato-Katz together, results were 94.73% and 93.58%, and using rapid sedimentation, Kato-Katz and copro-ELISA together, they were 97.56% and 100%, respectively. There was no correlation between coproantigen detection by optical density (OD) and infection intensity by eggs per gram of feces (epg) in Cajamarca low burden cases (
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0001812