Molecular cloning of the gene encoding developing seed L-asparaginase from Lupinus angustifolius

A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genom...

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Bibliographic Details
Published in:Plant molecular biology 1992-10, Vol.20 (2), p.333-336
Main Authors: Dickson, J.M.J.J. (Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand)), Vincze, E.V, Grant, M.R, Smith, L.A, Rodber, K.A, Farnden, K.J.F, Reynolds, P.H.S
Format: Article
Language:English
Subjects:
ADN
AMIDE HYDROLASE
AMIDE HYDROLASES
AMIDO HIDROLASA
Amino Acid Sequence
Asparaginase - genetics
Base Sequence
Biochemistry & Molecular Biology
Biological and medical sciences
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
CODE GENETIQUE
CODIGO GENETICO
DNA
Fundamental and applied biological sciences. Psychology
GENETIC CODE
Life Sciences & Biomedicine
LUPINUS ANGUSTIFOLIUS
Molecular and cellular biology
MOLECULAR CLONING
Molecular genetics
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Plant Sciences
Plants - embryology
Plants - enzymology
Plants - genetics
Science & Technology
SECUENCIA NUCLEICA
Seeds - enzymology
Seeds - genetics
Seeds - growth & development
SEQUENCE NUCLEIQUE
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Summary:A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00014503