An electrochemical immunosensor for ultrasensitive detection of carbohydrate antigen 199 based on Au@CuxOS yolk–shell nanostructures with porous shells as labels

A novel and sensitive electrochemical immunosensor for ultrasensitive detection of pancreatic cancer biomarker carbohydrate antigen 199 (CA199) was proposed by using Au@CuxOS yolk–shell nanostructures with porous shells as labels for signal amplification. Au@CuxOS yolk–shell nanostructures exhibit h...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-01, Vol.63, p.39-46
Main Authors: Guo, Aiping, Li, Yueyun, Cao, Wei, Meng, Xianchao, Wu, Dan, Wei, Qin, Du, Bin
Format: Article
Language:English
Subjects:
Au nanoparticles
Au@CuxOS yolk–shell nanostructures with porous shells
Biological and medical sciences
Biosensors
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Pancreatic cancer biomarker CA199
Reduced graphene oxide–tetraethylene pentamine (rGO–TEPA)
Various methods and equipments
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Summary:A novel and sensitive electrochemical immunosensor for ultrasensitive detection of pancreatic cancer biomarker carbohydrate antigen 199 (CA199) was proposed by using Au@CuxOS yolk–shell nanostructures with porous shells as labels for signal amplification. Au@CuxOS yolk–shell nanostructures exhibit high electrocatalytic activity toward the reduction of hydrogen peroxide (H2O2) as analytical signal. Moreover, secondary antibody (Ab2) can adsorb on the surface of Au@CuxOS with porous shells which has large surface area and could greatly increase the probability of Ab2–antigen interactions thereby leading to higher sensitivity. Reduced graphene oxide–tetraethylene pentamine (rGO–TEPA), containing abundant amine groups, was supported Au nanoparticles as a support platform to immobilize the primary antibody (Ab1). The resulting sensing interface of rGO–TEPA/AuNPs could provide a large electroconductive surface area, allowing high loadings of the biological recognition elements as well as the occurrence of electrocatalytic and electron-transfer processes. Under optimal conditions, the immunosensor exhibited a wide linear response to CA199 ranging from 0.001 to 12U/mL with a low detection limit of 0.0005U/mL. The designed immunosensor displayed good precision, high sensitivity, acceptable stability and reproducibility, and has been applied to the analysis of serum with satisfactory results. The proposed method provides a new promising platform of clinical immunoassay for other biomolecules. •A novel and sensitive electrochemical immunosensor for the detection of CA199 was developed.•Au@CuxOS yolk–shell nanostructures with porous shells were used as labels.•The electrocatalytic activity of Au@CuxOS toward H2O2 was monitored as analytical signal.•The immunosensor exhibited a wide linear from 0.001 to 12U/mL with a low detection limit of 0.0005U/mL.•Satisfactory results were obtained for determination of CA199 in human serum samples.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.07.017