Validation of radioimmunoassay screening methods for β-agonists in bovine liver according to Commission Decision 2002/657/EC

Validation studies were carried out on a multi-residue screening method for anilinic type β-agonists (clenbuterol, mabuterol, brombuterol, cimaterol, cimbuterol, mapenterol, clenpenterol) and a method for the phenolic type β-agonist, salbutamol, in bovine liver. The validation was performed accordin...

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Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2008-12, Vol.25 (12), p.1475-1481
Main Authors: Granja, R.H.M.M., Montes Niño, A.M., Rabone, F., Montes Niño, R.E., Cannavan, A., Gonzalez Salerno, A.
Format: Article
Language:English
Subjects:
Adrenergic beta-Agonists - analysis
Animals
Biological and medical sciences
Cattle
Drug Residues - analysis
Food Analysis - methods
Food Analysis - standards
Food Contamination - analysis
Food industries
Fundamental and applied biological sciences. Psychology
Liver - chemistry
Meat - analysis
Quality Control
radioimmunoassay
Radioimmunoassay - methods
residues
validation
β-agonists
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Summary:Validation studies were carried out on a multi-residue screening method for anilinic type β-agonists (clenbuterol, mabuterol, brombuterol, cimaterol, cimbuterol, mapenterol, clenpenterol) and a method for the phenolic type β-agonist, salbutamol, in bovine liver. The validation was performed according to the European Union Commission Decision 2002/657/EC (European Commission 2002), which establishes criteria and procedures for the determination of parameters such as the detection capability (CCβ), specificity, stability of standard solutions and stability of the analyte in matrix. CCβ values for the eight target compounds were between 0.25 and 0.5 µg kg −1 . The stability of standard solutions and analytes in matrix and the specificity of the antibody were characterized. The methods are applicable for qualitative screening of β-agonists for regulatory programmes according to European Union performance requirements, or as a semi-quantitative research tool for known target compounds.
ISSN:1944-0049
1944-0057
DOI:10.1080/02652030802308464