Global Transcriptome Analysis of Borrelia burgdorferi during Association with Human Neuroglial Cells

As adherence and entry of a pathogen into a host cell are key components to an infection, identifying the molecular mechanisms responsible for cellular association will provide a better understanding of a microbe's pathogenesis. We previously established an in vitro model for Borrelia burgdorfe...

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Bibliographic Details
Published in:Infection and Immunity 2008-01, Vol.76 (1), p.298-307
Main Authors: Livengood, Jill A, Schmit, Virginia L, Gilmore, Robert D. Jr
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Borrelia burgdorferi
Borrelia burgdorferi - genetics
Borrelia burgdorferi - metabolism
Cell Line
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Humans
Microbiology
Miscellaneous
Molecular Pathogenesis
Multigene Family
Neuroglia - cytology
Neuroglia - microbiology
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Summary:As adherence and entry of a pathogen into a host cell are key components to an infection, identifying the molecular mechanisms responsible for cellular association will provide a better understanding of a microbe's pathogenesis. We previously established an in vitro model for Borrelia burgdorferi infection of human neuroglial cells. To expand on our earlier study, we performed B. burgdorferi whole-genome expression analysis following a 20-hour infection of human neuroglial cells to identify borrelial genes that were differentially regulated during host-cell association compared with cultured Borrelia in cell-free medium. This study identifies several regulated genes, the products of which may be important mediators of cellular pathogenesis.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.00866-07