Characterization of Melanotropin-Release-Inhibiting Factor (Melanostatin) From Frog Brain: Homology with Human Neuropeptide Y

A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit α-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting fact...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS 1991-05, Vol.88 (9), p.3862-3866
Main Authors:	CHARTREL, N, CONLON, J. M, DANGER, J.-M, FOURNIER, A, TONON, M.-C, VAUDRY, H
Format:	Article
Language:	eng
Subjects:	alpha-MSH - metabolism Amino Acid Sequence Amino acids Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Biological and medical sciences brain Brain - metabolism Brain Chemistry Dose-Response Relationship, Drug Endocrinology Flow velocity Frogs Fundamental and applied biological sciences. Psychology Gels Molecular Sequence Data MSH Release-Inhibiting Hormone - chemistry MSH Release-Inhibiting Hormone - pharmacology neuropeptide Y Neuropeptides Physiology Proteins Rana ridibunda Ranidae Resins Secretory Rate - drug effects Sex hormones
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Summary:	A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit α-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting factor (melanostatin) was determined to be H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Met- Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg- Tyr-NH2. Frog melanostatin belongs to the pancreatic polypeptide/neuropeptide Y/peptide YY family, and the structure of this peptide differs from that of human neuropeptide Y by only one amino acid substitution in position 19. A synthetic replicate of frog melanostatin is coeluted with the native peptide on HPLC and is highly potent in inhibiting α-melanotropin secretion in vitro (IC50= 60 nM).
ISSN:	0027-8424 1091-6490