Simultaneous Determination of Creatine and Creatinine using Monocrystalline Diamond Paste-Based Amperometric Biosensors

In order to determine creatine and creatinine, amperometric diamond paste biosensors were proposed. A bienzymatic biosensor based on creatinase and sarcosine oxidase was used for the assay of creatine and a trienzymatic biosensor based on creatinase, sarcosine oxidase, and creatininase was proposed...

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Bibliographic Details
Published in:Analytical letters 2006-08, Vol.39 (11), p.2227-2233
Main Authors: Staden, Raluca-Ioana Stefan-van, Bokretsion, Rahel Girmai
Format: Article
Language:English
Subjects:
amperometric biosensors
Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
creatinase
Creatine
creatininase
creatinine
Electrochemical methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Methods. Procedures. Technologies
sorcosine oxidase
Various methods and equipments
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Summary:In order to determine creatine and creatinine, amperometric diamond paste biosensors were proposed. A bienzymatic biosensor based on creatinase and sarcosine oxidase was used for the assay of creatine and a trienzymatic biosensor based on creatinase, sarcosine oxidase, and creatininase was proposed for the assay of creatinine. The linear concentration ranges are of fmol/L magnitude order, with very low limits of detection. The biosensors proved to be highly reliable for determination of creatine and creatinine as raw materials in pharmaceutical formulations as well as in serum samples.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710600751016