Bacterial Ghosts as an Oral Vaccine: a Single Dose of Escherichia coli O157:H7 Bacterial Ghosts Protects Mice against Lethal Challenge

Enterohemorrhagic Escherichia coli (EHEC) is a bacterial pathogen that is associated with several life-threatening diseases for humans. The combination of protein E-mediated cell lysis to produce EHEC ghosts and staphylococcal nuclease A to degrade DNA was used for the development of an oral EHEC va...

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Bibliographic Details
Published in:Infection and Immunity 2005-08, Vol.73 (8), p.4810-4817
Main Authors: Mayr, Ulrike Beate, Haller, Christoph, Haidinger, Wolfgang, Atrasheuskaya, Alena, Bukin, Eugenij, Lubitz, Werner, Ignatyev, Georgy
Format: Article
Language:eng ; rus
Subjects:
Animals
Cell Membrane - immunology
Cell Proliferation
Colon - immunology
Escherichia coli
Escherichia coli Infections - immunology
Escherichia coli Infections - mortality
Escherichia coli Infections - prevention & control
Escherichia coli O157 - immunology
Escherichia coli O157 - ultrastructure
Immunoglobulin A - blood
Immunoglobulin G - blood
Interferon-gamma - metabolism
Mice
Microbial Immunity and Vaccines
Spleen - immunology
Spleen - metabolism
Vaccines, Subunit - immunology
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Summary:Enterohemorrhagic Escherichia coli (EHEC) is a bacterial pathogen that is associated with several life-threatening diseases for humans. The combination of protein E-mediated cell lysis to produce EHEC ghosts and staphylococcal nuclease A to degrade DNA was used for the development of an oral EHEC vaccine. The lack of genetic material in the oral EHEC bacterial-ghost vaccine abolished any hazard of horizontal gene transfer of resistance genes or pathogenic islands to resident gut flora. Intragastric immunization of mice with EHEC ghosts without the addition of any adjuvant induced cellular and humoral immunity. Immunized mice challenged at day 55 showed 86% protection against lethal challenge with a heterologous EHEC strain after single-dose oral immunization and 93.3% protection after one booster at day 28, whereas the controls showed 26.7% and 30% survival, respectively. These results indicate that it is possible to develop an efficacious single-dose oral EHEC bacterial-ghost vaccine.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.8.4810-4817.2005