Detection of Cryptosporidium parvum in soil extracts

Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on a...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2004-03, Vol.70 (3), p.1827-1829
Main Authors: Walker, M, Redelman, D
Format: Article
Language:English
Subjects:
animal parasites and pests
Animals
Antibodies, Protozoan
Biological and medical sciences
Cryptosporidium parvum
Cryptosporidium parvum - immunology
Cryptosporidium parvum - isolation & purification
Epifluorescence microscopy
epifluorescent microscopy
Flow Cytometry
fluorescein isothiocyanate
Fluorescein-5-isothiocyanate
fluorescent antibody technique
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
immunoglobulin G
immunoglobulin G3
immunoglobulin M
microbial detection
Microbiology
Microscopy, Fluorescence
oocysts
Oocysts - isolation & purification
Public health
Public Health Microbiology
sandy soils
Scientific apparatus & instruments
Soil - parasitology
soil pollution
Soils
Viruses
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Summary:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.70.3.1827-1829.2004