Potentiation of nerve growth factor-induced neurite outgrowth in PC12 cells by a coptidis rhizoma extract and protoberberine alkaloids

A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells i...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-11, Vol.66 (11), p.2491-2494
Main Authors: Shigeta, K. (Hiroshima Prefectural Univ., Shobara (Japan)), Ootaki, K, Tatemoto, H, Nakanishi, T, Inada, A, Muto, N
Format: Article
Language:English
Subjects:
ALKALOIDS
Animals
Berberine - analogs & derivatives
Berberine - pharmacology
Berberine Alkaloids - isolation & purification
Berberine Alkaloids - pharmacology
Biological and medical sciences
CELLS
Cholinesterase Inhibitors - pharmacology
Chromatography, High Pressure Liquid - methods
Coptidis Rhizoma
Dose-Response Relationship, Drug
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - pharmacology
Enzyme Activation - physiology
General pharmacology
Medical sciences
nerve growth factor
Nerve Growth Factors - pharmacology
NERVOUS SYSTEM
neurite outgrowth
Neurites - drug effects
Neurites - physiology
PC12 cell
PC12 Cells
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
protoberberine alkaloid
RANUNCULACEAE
RATS
RHIZOMES
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A methanol extract of Coptidis Rhizoma effectively enhanced the outgrowth of neurite in PC12 cells induced by nerve growth factor (NGF). Following solvent partition and preparative HPLC, berberine was isolated as the major active compound. Berberine enhanced the proportion of neurite-bearing cells in a dose-dependent manner without cytotoxicity. Its structural relatives, palmatine and coptisine, showed a slightly weaker NGF-enhancing effect than berberine. These three alkaloids inhibited acetylcholinesterase activity at a level comparable to that of physostigmine, but this inhibition was not responsible for the potentiation of NGF-induced neurite outgrowth. It is demonstrated for the first time that protoberberine alkaloids potentiated the NGF-induced differentiation of neural cells.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.66.2491