Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria

Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is ins...

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Bibliographic Details
Published in:Biochemistry (Easton) 2008-12, Vol.47 (49), p.12939-12941
Main Authors: Summerfield, Tina C., Toepel, Jörg, Sherman, Louis A.
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
CYANOBACTERIA
Cyanobacteria - genetics
Cyanobacteria - metabolism
Environmental Molecular Sciences Laboratory
FUNCTIONALS
Gene Expression Regulation, Bacterial
GENES
INDUCTION
Microarray Analysis
OXYGEN
Photosystem II Protein Complex - biosynthesis
Photosystem II Protein Complex - genetics
PROTEINS
RNA, Messenger - genetics
RNA, Messenger - metabolism
STRAINS
Synechocystis
Transcription, Genetic
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Summary:Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi8018916