Coenzyme F430 as a possible catalyst for the reductive dehalogenation of chlorinated C1 hydrocarbons in methanogenic bacteria

Corrinoids, such as aquocobalamin, methylcobalamin, and (cyanoaquo)cobinamide, catalyze the reductive dehalogenation of CCl4 with titanium(III) citrate as the electron donor [Krone et al. (1989) Biochemistry 28, 4908-4914]. We report here that this reaction is also effectively mediated by the nickel...

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Bibliographic Details
Published in:Biochemistry (Easton) 1989-12, Vol.28 (26), p.10061-10065
Main Authors: Krone, Ute E, Laufer, Kerstin, Thauer, Rudolf K, Hogenkamp, Harry P. C
Format: Article
Language:English
Subjects:
560300 - Chemicals Metabolism & Toxicology
ALKANES
BACTERIA
BARYONS
BIODEGRADATION
CARBON TETRACHLORIDE
Carbon Tetrachloride - metabolism
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CHLOROFORM
COENZYMES
DECOMPOSITION
Disulfides - metabolism
ELEMENTARY PARTICLES
ELEMENTS
ETHANE
Euryarchaeota - enzymology
FERMIONS
GROWTH
HADRONS
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
Metalloporphyrins
METALLOPROTEINS
Metalloproteins - metabolism
METALS
METHANE
Methane - metabolism
METHANOGENIC BACTERIA
METHYL CHLORIDE
METHYLENE CHLORIDE
MICROORGANISMS
NICKEL
Nickel - metabolism
NUCLEONS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
Oxidation-Reduction
PROTEINS
PROTONS
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
TITANIUM
Titanium - metabolism
TRANSITION ELEMENTS
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Summary:Corrinoids, such as aquocobalamin, methylcobalamin, and (cyanoaquo)cobinamide, catalyze the reductive dehalogenation of CCl4 with titanium(III) citrate as the electron donor [Krone et al. (1989) Biochemistry 28, 4908-4914]. We report here that this reaction is also effectively mediated by the nickel-containing porphinoid, coenzyme F430, found in methanogenic bacteria. Chloroform, methylene chloride, methyl chloride, and methane were detected as intermediates and products. Ethane was formed in trace amounts, and several as yet unidentified nonvolatile compounds were also generated. The rate of dehalogenation decreased in the series of CCl4, CHCl3, and CH2Cl2. With coenzyme F430 as the catalyst, the reduction of CH3Cl to CH4 proceeded more than 50 times faster than with aquocobalamin. Cell suspensions of Methanosarcina barkeri were found to catalyze the reductive dehalogenation of CCl4 with CO as the electron donor (E'0 = -0.524 V). Methylene chloride was the main end product. The kinetics of CHCl3 and CH2Cl2 formation from CCl4 were similar to those with coenzyme F430 or aquocobalamin as catalysts and titanium(III) citrate as the reductant.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00452a027