Usefulness of fibroblast culture for testing of cattle tissues polluted with heavy metals

Cattle tissues (liver, kidney, brain, and lung) that had been polluted with heavy metals were tested for their ability to alter fibroblast culture growth, cellular protein and DNA content, and fibroblast DNA synthesis. At 72 hr of incubation a significant increase in cellular DNA and [ 14C]thymidine...

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Bibliographic Details
Published in:Environmental research 1990-04, Vol.51 (2), p.163-169
Main Authors: Wȩglarz, Ludmiła, Dróżdż, Marian, Wardas, Maria, Kula, Bogusław, Pawlaczyk-Szpilowa, Maria
Format: Article
Language:English
Subjects:
550501 > Metabolism-- Tracer Techniques
ANIMAL CELLS
ANIMALS
AZINES
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
CARBON 14 COMPOUNDS
Carbon Radioisotopes
CATTLE
CELL CULTURES
CELL DIVISION
Cell Division - drug effects
Cells, Cultured
CONNECTIVE TISSUE CELLS
CONTAMINATION
DISTRIBUTION
DNA
DNA - analysis
DNA - biosynthesis
DNA REPLICATION
DOMESTIC ANIMALS
ELEMENTS
Environmental pollutants toxicology
FIBROBLASTS
Fibroblasts - analysis
Fibroblasts - drug effects
Fibroblasts - metabolism
FOOD CHAINS
General aspects
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
Medical sciences
METALS
Metals - analysis
Metals - pharmacokinetics
Metals - pharmacology
MICE
Mice, Inbred BALB C
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RIBOSIDES
RODENTS
RUMINANTS
Soil Pollutants - analysis
SOILS
SOMATIC CELLS
THYMIDINE
Thymidine - metabolism
TISSUE DISTRIBUTION
Toxicology
TRACER TECHNIQUES
VERTEBRATES 560300 > Chemicals Metabolism & Toxicology
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Summary:Cattle tissues (liver, kidney, brain, and lung) that had been polluted with heavy metals were tested for their ability to alter fibroblast culture growth, cellular protein and DNA content, and fibroblast DNA synthesis. At 72 hr of incubation a significant increase in cellular DNA and [ 14C]thymidine incorporation was noted in the primary cultures as well as in the subcultures compared to controls. Fibroblast cultures also displayed growth inhibition and reduction in protein content. The measurement of basic biochemical parameters of the fibroblast culture may represent a sensitive means of assessing rapidly the activity of heavy metals deposited in the tissues of cattle as a result of their grazing on polluted soil.
ISSN:0013-9351
1096-0953
DOI:10.1016/S0013-9351(05)80086-6