Flow Cytometric Analysis of Expression of Interleukin-2 Receptor β Chain (p70-75) on Various Leukemic Cells

We analyzed the expression of the interleukin-2 receptor (IL-2R) β chain (p70-75) on various leukemic cells from 44 patients by flow cytometric analysis using the IL-2Rβ chain-specific monoclonal antibody (MoAb), designated Mik-β1, which has been recently developed. Flow cytometric analysis demonstr...

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Bibliographic Details
Published in:Blood 1990-08, Vol.76 (4), p.767-774
Main Authors: Hoshino, Shigeru, Oshimi, Kazuo, Tsudo, Mitsuru, Miyasaka, Masayuki, Teramura, Masanao, Masuda, Michihiko, Motoji, Toshiko, Mizoguchi, Hideaki
Format: Article
Language:English
Subjects:
550901 > Pathology-- Tracer Techniques
ANIMAL CELLS
ANIMALS
ANTIBODIES
Antibodies, Monoclonal - immunology
Antibody Affinity - immunology
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
Biological and medical sciences
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
Flow Cytometry
Gene Expression
GENE REGULATION
GROWTH FACTORS
Hematologic and hematopoietic diseases
HEMIC DISEASES
Humans
IMMUNE SYSTEM DISEASES
Interleukin-2 - immunology
Interleukin-2 - metabolism
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
Iodine Radioisotopes
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKEMIA
Leukemia - blood
Leukemia - metabolism
Leukemia - pathology
Leukemia, Lymphocytic, Chronic, B-Cell - blood
Leukemia, Lymphocytic, Chronic, B-Cell - metabolism
Leukemia, Lymphocytic, Chronic, B-Cell - pathology
Leukemia, Myeloid, Acute - blood
Leukemia, Myeloid, Acute - metabolism
Leukemia, Myeloid, Acute - pathology
Leukemia, T-Cell - blood
Leukemia, T-Cell - metabolism
Leukemia, T-Cell - pathology
Leukemia-Lymphoma, Adult T-Cell - blood
Leukemia-Lymphoma, Adult T-Cell - metabolism
Leukemia-Lymphoma, Adult T-Cell - pathology
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
LEUKOCYTES
LYMPHOCYTES
Lymphocytes - immunology
Lymphocytes - metabolism
Lymphocytes - ultrastructure
LYMPHOKINES
Lymphoproliferative Disorders - blood
Lymphoproliferative Disorders - metabolism
Lymphoproliferative Disorders - pathology
MAMMALS
MAN
MATERIALS
Medical sciences
MEMBRANE PROTEINS
MITOGENS
MONOCLONAL ANTIBODIES
Multiple Myeloma - blood
Multiple Myeloma - metabolism
Multiple Myeloma - pathology
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood
Precursor Cell Lymphoblastic Leukemia-Lymphoma - metabolism
Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
Receptors, Interleukin-2 - analysis
Receptors, Interleukin-2 - genetics
Receptors, Interleukin-2 - immunology
Receptors, Interleukin-2 - metabolism
SOMATIC CELLS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES 550201 > Biochemistry-- Tracer Techniques
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Summary:We analyzed the expression of the interleukin-2 receptor (IL-2R) β chain (p70-75) on various leukemic cells from 44 patients by flow cytometric analysis using the IL-2Rβ chain-specific monoclonal antibody (MoAb), designated Mik-β1, which has been recently developed. Flow cytometric analysis demonstrated the expression of the IL-2Rβ chain on granular lymphocytes (GLs) from all eight patients with granular lymphocyte proliferative disorders (GLPDs), on adult T-cell leukemia (ATL) cells from all three patients with ATL, and on T-cell acute lymphoblastic leukemia (T-ALL) cells from one of three patients with T-ALL. Although GLs from all the GLPD patients expressed the IL-2Rβ chain alone and not the IL-2Rα chain (Tac-antigen: p55), ATL and T-ALL cells expressing the β chain coex-pressed the α chain. In two of seven patients with common ALL (cALL) and in both patients with B-cell chronic lymphocytic leukemia, the leukemic cells expressed the α chain alone. Neither the α chain nor the β chain was expressed on leukemic cells from the remaining 28 patients, including all 18 patients with acute nonlymphocytic leukemia, five of seven patients with cALL, all three patients with multiple myeloma, and two of three patients with T-ALL. These results indicate that three different forms of IL-2R chain expression exist on leukemic cells: the α chain alone; the β chain alone; and both the α and β chains. To examine whether the results obtained by flow cytometric analysis actually reflect functional aspects of the expressed IL-2Rs, we studied the specific binding of 126l-labeled IL-2 (125l-IL-2) to leukemic cells in 18 of the 44 patients. In addition, we performed 125l—IL-2 crosslinking studies in seven patients. The results of IL-2R expression of both 125l-IL-2 binding assay and crosslinking studies were in agreement with those obtained by flow cytometric analysis. These results indicate that flow cytometric analysis using MoAbs, anti-Tac, and Mik-β 1 is useful for detecting the expression of the IL-2R chains.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V76.4.767.767