Isolation and characterization of the hamster gadd153 gene. Activation of promoter activity by agents that damage DNA

A group of five cDNA clones, representing the gadd genes, were recently isolated from Chinese hamster ovary (CHO) cells as genes induced upon growth arrest and after DNA damage (Fornace, A. J., Jr., Nebert, D. W., Hollander, M. C., Luethy, J. D., Papathanasiou, M., Fargnoli, J., and Holbrook, N. J....

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1990-09, Vol.265 (27), p.16521-16526
Main Authors: Luethy, J D, Fargnoli, J, Park, J S, Fornace, A J, Holbrook, N J
Format: Article
Language:English
Subjects:
560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture
560300 - Chemicals Metabolism & Toxicology
ANIMAL CELLS
ANIMALS
Base Sequence
Biological and medical sciences
Cell Line
CHO CELLS
Cricetinae
Cricetulus
DNA
DNA Damage
ELECTROMAGNETIC RADIATION
ESTERS
Exons
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - drug effects
Gene Expression Regulation - radiation effects
Gene Library
GENE REGULATION
Genes. Genome
GENETIC MAPPING
HAMSTERS
HeLa Cells - drug effects
HeLa Cells - metabolism
Humans
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
Hydrogen Peroxide - pharmacology
Introns
MAMMALS
MAPPING
METHYL METHANESULFONATE
Methyl Methanesulfonate - pharmacology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
MUTAGENESIS
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
Promoter Regions, Genetic - drug effects
Promoter Regions, Genetic - radiation effects
Prostaglandins A - pharmacology
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
Restriction Mapping
RODENTS
SULFONIC ACID ESTERS
Tetradecanoylphorbol Acetate - pharmacology
Transcriptional Activation
ULTRAVIOLET RADIATION
Ultraviolet Rays
VERTEBRATES
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A group of five cDNA clones, representing the gadd genes, were recently isolated from Chinese hamster ovary (CHO) cells as genes induced upon growth arrest and after DNA damage (Fornace, A. J., Jr., Nebert, D. W., Hollander, M. C., Luethy, J. D., Papathanasiou, M., Fargnoli, J., and Holbrook, N. J. (1989) Mol. Cell. Biol. 9, 4196-4203). We have isolated and characterized one of these genes, gadd153. The gene spans five kilobases and contains four exons. The 5'-flanking region of the gene, within 420 base pairs of the transcription initiation site, contains a number of cis elements associated with transcriptional regulation in other genes. These include a Hogness box, ATAAAA, an inverted GCCAAT box; seven SP1 transcription factor binding sites, and an AP-1 site. This region is rich in G + C content (greater than 70%) and contains an unusually long stretch of alternating CpG residues. The 800-base pair region immediately upstream of the transcription start site can drive expression of the bacterial chloramphenicol acetyltransferase (CAT) gene, but only in its endogenous orientation, in three different cell lines: HeLa, CHO, and Jurkat. The gadd153 promoter is strongly activated by methyl methanesulfonate, hydrogen peroxide, and UV irradiation, but not by growth arrest signals. This suggests that separate and very different regulatory pathways are involved in the induction of the gadd153 gene by growth cessation and DNA damage.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)46254-5