Encapsulation of the UV-DNA Repair Enzyme T4 Endonuclease V in Liposomes and Delivery to Human Cells

T4 endonuclease V, a pyrimidine-dimer-specific DNA repair enzyme, was encapsulated in liposomes under mild conditions. The encapsulated enzyme was active, and when applied to ultraviolet (UV)-irradiated human cells in culture, the liposomes increased incision of UV-irradiated cellular DNA, enhanced...

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Bibliographic Details
Published in:Journal of investigative dermatology 1989-08, Vol.93 (2), p.190-194
Main Authors: Ceccoli, Joseph, Rosales, Nestor, Tsimis, Jeannie, Yarosh, Daniel B
Format: Article
Language:English
Subjects:
560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture
Analytical, structural and metabolic biochemistry
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
Biological and medical sciences
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
Biotechnology
BODY
CELL CONSTITUENTS
CELL CULTURES
Cell Line, Transformed
Cell Survival
Cells, Cultured
CONNECTIVE TISSUE CELLS
DNA
DNA - drug effects
DNA - radiation effects
DNA Damage
DNA REPAIR
DNA-ASE
Drug Carriers
ELECTROMAGNETIC RADIATION
ENCAPSULATION
Endodeoxyribonucleases - administration & dosage
Endodeoxyribonucleases - pharmacology
ENDONUCLEASES
ENZYMES
Enzymes and enzyme inhibitors
Epidermal Cells
EPIDERMIS
Epidermis - drug effects
Epidermis - radiation effects
EPITHELIUM
ESTERASES
FIBROBLASTS
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - radiation effects
Fundamental and applied biological sciences. Psychology
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
Humans
HYDROLASES
Immobilization of enzymes and other molecules
Immobilization techniques
KERATIN
Keratins
LIPOSOMES
MAMMALS
MAN
Methods. Procedures. Technologies
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHODIESTERASES
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
RECOVERY
REPAIR
SCLEROPROTEINS
SKIN
SOMATIC CELLS
SURVIVAL TIME
TISSUES
ULTRAVIOLET RADIATION
Ultraviolet Rays
VERTEBRATES
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Summary:T4 endonuclease V, a pyrimidine-dimer-specific DNA repair enzyme, was encapsulated in liposomes under mild conditions. The encapsulated enzyme was active, and when applied to ultraviolet (UV)-irradiated human cells in culture, the liposomes increased incision of UV-irradiated cellular DNA, enhanced DNA repair replication, and enhanced survival of UV-irradiated cells. This method is a first step in a new approach for topical application of DNA repair enzymes to human skin to prevent skin cancer.
ISSN:0022-202X
1523-1747
DOI:10.1111/1523-1747.ep12277569