Harmine promotes molar root development via SMAD1/5/8 phosphorylation

Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiation in vitro and in vivo. Here we examined th...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-03, Vol.497 (3), p.924-929
Main Authors: Fujiwara, Naoki, Lee, Ji-Won, Kumakami-Sakano, Mika, Otsu, Keishi, Woo, Je-Tae, Iseki, Sachiko, Ota, Masato S.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
BIOLOGICAL EFFECTS
Cell movement
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
Hertwig's epithelial root sheath
Natural small compound
PHOSPHORYLATION
Postnatal development
SKELETON
TEETH
TISSUE CULTURES
Tooth root
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Summary:Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiation in vitro and in vivo. Here we examined the biological effect of HMN on the postnatal development of molar tooth roots and periodontal tissues. HMN supported the formation of tooth roots and periodontal tissues in developing tooth germs. In tooth germ organ culture, HMN promoted the elongation of Hertwig's epithelial root sheath (HERS) and stimulated cell proliferation in HERS and dental follicle-derived tissues, including dental papillae and dental follicles. HMN stimulated cell proliferation and cell movement of HERS-derived cells without mesenchymal cells in vitro and directly induced the phosphorylation of SMAD1/5/8 protein in HERS-derived cells. Our results indicated that HMN was the first natural small compound to stimulate postnatal development of tooth germs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2017.12.062