Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring

Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal biomarker for eco-monitoring of polluted areas. Electron spin resonance (ESR) is the most definitive method for detecting, quantifying and possibly identifying radicals in complex systems. A non-invasive...

Full description

Saved in:
Bibliographic Details
Published in:Environmental research 2018-08, Vol.165, p.11-18
Main Authors: D’Errico, Gerardino, Vitiello, Giuseppe, De Tommaso, Gaetano, Abdel-Gawad, Fagr Kh, Brundo, Maria Violetta, Ferrante, Margherita, De Maio, Anna, Trocchia, Samantha, Bianchi, Anna Rita, Ciarcia, Gaetano, Guerriero, Giulia
Format: Article
Language:English
Subjects:
ANIMAL TISSUES
BIOLOGICAL MARKERS
BIOPSY
CONCENTRATION RATIO
DETOXIFICATION
ECOLOGICAL CONCENTRATION
ELECTRON SPIN RESONANCE
ENVIRONMENTAL SCIENCES
FROGS
LIVER
MONITORING
Poly(ADPribose) polymerase
POLYMERASES
Reactive oxygen species
SKIN
Skin biopsy
SPECTROSCOPY
SPIN
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal biomarker for eco-monitoring of polluted areas. Electron spin resonance (ESR) is the most definitive method for detecting, quantifying and possibly identifying radicals in complex systems. A non-invasive method for monitoring polluted areas by the quantitative determination of ROS in frog skin biopsy is presented. We assessed by ESR spectroscopy the ROS level in adult male of Pelophylax bergeri, specie not a risk of extinction, collected from the polluted Sarno River (SA, Italy) basin. The spin-trap ESR method was validated by immunohistochemical analysis of the well-assessed pollution biomarkers cytochrome P450 aromatase 1A (CYP1A) and glutathione S-transferase (GST), and by determining the poly(ADPribose) polymerase (PARP) and GST enzymatic activity. ROS concentration in skin samples from frogs collected in the polluted area is significantly higher than that determined for the unpolluted reference area. Immunohistochemical analysis of CYP1A and GST supported the reliability of our approach, even in the absence of evident morphological and ultrastructural differences. PARP activity assay, connected to possible oxidative DNA damage, and the detoxification index by GST enzymatic assay give statistically significant evidence that higher levels of ROS are associated to alterations of the different biomarkers. ROS concentration, measured by ESR on isolated frog skin, through the presented non-lethal method, is a reliable biomarker for toxicity screening and represents a useful basic datum for future modelling studies on environmental monitoring and biodiversity loss prevention. •ROS levels in frog skin biopsy were determined by spin-trapping ESR spectroscopy.•Skin of frogs collected in polluted areas contains higher ROS concentration.•ROS levels determined in skin biopsy follow the same trend of those from liver samples.•Immunohistochemical analyses and enzymatic activity tests validate the method.•ROS concentration determined by ESR in biosentinel biopsy is an effective biomarker.
ISSN:0013-9351
1096-0953
DOI:10.1016/j.envres.2018.03.044