Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes

Although mast cells are traditionally thought to function as effector cells in allergic responses, they have increasingly been recognized as important regulators of various immune responses. Mast cells mature locally; thus, tissue-specific influences are important for promoting mast cell accumulatio...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2017-06, Vol.487 (4), p.856-861
Main Authors: Cho, Kyung-Ah, Park, Minhwa, Kim, Yu-Hee, Woo, So-Youn
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ANIMAL TISSUES
Cell Line
CELL PROLIFERATION
GASTROINTESTINAL TRACT
GROWTH FACTORS
Humans
INFLAMMATION
Keratinocyte
Keratinocytes - metabolism
Mast cell
MAST CELLS
Mast Cells - cytology
Mast Cells - immunology
PLANT GROWTH
Psoriasis
SKIN
Stem cell factor
Stem Cell Factor - metabolism
STEM CELLS
Th17 Cells - immunology
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Summary:Although mast cells are traditionally thought to function as effector cells in allergic responses, they have increasingly been recognized as important regulators of various immune responses. Mast cells mature locally; thus, tissue-specific influences are important for promoting mast cell accumulation and survival in the skin and the gastrointestinal tract. In this study, we determined the effects of keratinocytes on mast cell accumulation during Th17-mediated skin inflammation. We observed increases in dermal mast cells in imiquimod-induced psoriatic dermatitis in mice accompanied by the expression of epidermal stem cell factor (SCF), a critical mast cell growth factor. Similar to mouse epidermal keratinocytes, SCF was highly expressed in the human HaCaT keratinocyte cell line following stimulation with IL−17. Further, keratinocytes promoted mast cell proliferation following stimulation with IL−17 in vitro. However, the effects of keratinocytes on mast cells were significantly diminished in the presence of anti−CD117 (stem cell factor receptor) blocking antibodies. Taken together, our results revealed that the Th17-mediated inflammatory environment promotes mast cell accumulation through keratinocyte-derived SCF. •Psoriasis-like skin inflammation increase dermal mast cells.•Keratinocyte produce stem cell factor in psoriasis-like skin inflammation.•Keratinocyte promote mast cell proliferation by stem cell factor dependent manner
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2017.04.141