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Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells
•Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate a...
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| Published in: | Biochemical and biophysical research communications 2014-08, Vol.450 (4), p.1593-1599 |
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| cited_by | cdi_FETCH-LOGICAL-c299t-cd62ff885a2679184a4de107cf103827ac5aef788bc37106fab9d61b6c126dc3 |
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| cites | cdi_FETCH-LOGICAL-c299t-cd62ff885a2679184a4de107cf103827ac5aef788bc37106fab9d61b6c126dc3 |
| container_end_page | 1599 |
| container_issue | 4 |
| container_start_page | 1593 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 450 |
| creator | Kim, Yong Il Ryu, Jae-Sung Yeo, Jee Eun Choi, Yun Jin Kim, Yong Sang Ko, Kinarm Koh, Yong-Gon |
| description | •Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate and accelerate chondrogenesis.
Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs. |
| doi_str_mv | 10.1016/j.bbrc.2014.07.045 |
| format | article |
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Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2014.07.045</identifier><identifier>PMID: 25035928</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; BIOLOGICAL MARKERS ; Cartilage - cytology ; Cell Differentiation ; CELL PROLIFERATION ; Cells, Cultured ; Chondrogenic differentiation ; COMPARATIVE EVALUATIONS ; DNA ; Flow Cytometry ; GENES ; GROWTH FACTORS ; HUMAN POPULATIONS ; Humans ; PHENOTYPE ; POLYMERASE CHAIN REACTION ; Proliferation ; Reverse Transcriptase Polymerase Chain Reaction ; STEM CELLS ; Stem Cells - cytology ; Synovial Membrane - cytology ; Synovium-derived mesenchymal stem cell ; TGF-β ; TOLUIDINE BLUE ; TRANSCRIPTION FACTORS ; Transduction, Genetic ; Transforming Growth Factor beta1 - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2014-08, Vol.450 (4), p.1593-1599</ispartof><rights>2014</rights><rights>Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-cd62ff885a2679184a4de107cf103827ac5aef788bc37106fab9d61b6c126dc3</citedby><cites>FETCH-LOGICAL-c299t-cd62ff885a2679184a4de107cf103827ac5aef788bc37106fab9d61b6c126dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,786,790,891,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25035928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22416700$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yong Il</creatorcontrib><creatorcontrib>Ryu, Jae-Sung</creatorcontrib><creatorcontrib>Yeo, Jee Eun</creatorcontrib><creatorcontrib>Choi, Yun Jin</creatorcontrib><creatorcontrib>Kim, Yong Sang</creatorcontrib><creatorcontrib>Ko, Kinarm</creatorcontrib><creatorcontrib>Koh, Yong-Gon</creatorcontrib><title>Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>•Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate and accelerate chondrogenesis.
Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>BIOLOGICAL MARKERS</subject><subject>Cartilage - cytology</subject><subject>Cell Differentiation</subject><subject>CELL PROLIFERATION</subject><subject>Cells, Cultured</subject><subject>Chondrogenic differentiation</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>DNA</subject><subject>Flow Cytometry</subject><subject>GENES</subject><subject>GROWTH FACTORS</subject><subject>HUMAN POPULATIONS</subject><subject>Humans</subject><subject>PHENOTYPE</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Proliferation</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>STEM CELLS</subject><subject>Stem Cells - cytology</subject><subject>Synovial Membrane - cytology</subject><subject>Synovium-derived mesenchymal stem cell</subject><subject>TGF-β</subject><subject>TOLUIDINE BLUE</subject><subject>TRANSCRIPTION FACTORS</subject><subject>Transduction, Genetic</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhi0EokvhBTigSFy4JIydxE4kLqiiLVKlXvbAzXLsMevVxl7sJGpfiwfhmXCUwrGnkUbf_-uf-Ql5T6GiQPnnYzUMUVcMaFOBqKBpX5AdhR5KRqF5SXYAwEvW0x8X5E1KRwBKG96_JheshbrtWbcjy_2CER_OEVNywRfBFvub6_LPb1qgPyivMRX6ELyJ4Sd6pwvjrM0KPzk1rQLlTXGO4eTydttki8M8Kl-kRx8WN4-lwegWNEWacCw0nk7pLXll1Snhu6d5SfbX3_ZXt-Xd_c33q693pWZ9P5XacGZt17WKcdHTrlGNQQpCWwp1x4TSrUIrum7QtaDArRp6w-nANWXc6PqSfNxsQ5qcTNpNqA86eI96kow1lAuATH3aqHzHrxnTJEeX1pjKY5iTpG1bCxAd9BllG6pjSCmilefoRhUfJQW5liKPci1FrqVIEDKXkkUfnvznYUTzX_KvhQx82QDMr1gcxjUq5t8bF9ekJrjn_P8COpagGA</recordid><startdate>20140808</startdate><enddate>20140808</enddate><creator>Kim, Yong Il</creator><creator>Ryu, Jae-Sung</creator><creator>Yeo, Jee Eun</creator><creator>Choi, Yun Jin</creator><creator>Kim, Yong Sang</creator><creator>Ko, Kinarm</creator><creator>Koh, Yong-Gon</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20140808</creationdate><title>Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells</title><author>Kim, Yong Il ; Ryu, Jae-Sung ; Yeo, Jee Eun ; Choi, Yun Jin ; Kim, Yong Sang ; Ko, Kinarm ; Koh, Yong-Gon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-cd62ff885a2679184a4de107cf103827ac5aef788bc37106fab9d61b6c126dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>BIOLOGICAL MARKERS</topic><topic>Cartilage - cytology</topic><topic>Cell Differentiation</topic><topic>CELL PROLIFERATION</topic><topic>Cells, Cultured</topic><topic>Chondrogenic differentiation</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>DNA</topic><topic>Flow Cytometry</topic><topic>GENES</topic><topic>GROWTH FACTORS</topic><topic>HUMAN POPULATIONS</topic><topic>Humans</topic><topic>PHENOTYPE</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Proliferation</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>STEM CELLS</topic><topic>Stem Cells - cytology</topic><topic>Synovial Membrane - cytology</topic><topic>Synovium-derived mesenchymal stem cell</topic><topic>TGF-β</topic><topic>TOLUIDINE BLUE</topic><topic>TRANSCRIPTION FACTORS</topic><topic>Transduction, Genetic</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yong Il</creatorcontrib><creatorcontrib>Ryu, Jae-Sung</creatorcontrib><creatorcontrib>Yeo, Jee Eun</creatorcontrib><creatorcontrib>Choi, Yun Jin</creatorcontrib><creatorcontrib>Kim, Yong Sang</creatorcontrib><creatorcontrib>Ko, Kinarm</creatorcontrib><creatorcontrib>Koh, Yong-Gon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yong Il</au><au>Ryu, Jae-Sung</au><au>Yeo, Jee Eun</au><au>Choi, Yun Jin</au><au>Kim, Yong Sang</au><au>Ko, Kinarm</au><au>Koh, Yong-Gon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2014-08-08</date><risdate>2014</risdate><volume>450</volume><issue>4</issue><spage>1593</spage><epage>1599</epage><pages>1593-1599</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>•Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate and accelerate chondrogenesis.
Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25035928</pmid><doi>10.1016/j.bbrc.2014.07.045</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2014-08, Vol.450 (4), p.1593-1599 |
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| source | ScienceDirect Freedom Collection |
| subjects | 60 APPLIED LIFE SCIENCES BIOLOGICAL MARKERS Cartilage - cytology Cell Differentiation CELL PROLIFERATION Cells, Cultured Chondrogenic differentiation COMPARATIVE EVALUATIONS DNA Flow Cytometry GENES GROWTH FACTORS HUMAN POPULATIONS Humans PHENOTYPE POLYMERASE CHAIN REACTION Proliferation Reverse Transcriptase Polymerase Chain Reaction STEM CELLS Stem Cells - cytology Synovial Membrane - cytology Synovium-derived mesenchymal stem cell TGF-β TOLUIDINE BLUE TRANSCRIPTION FACTORS Transduction, Genetic Transforming Growth Factor beta1 - metabolism |
| title | Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells |
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