Overexpression of TGF-β1 enhances chondrogenic differentiation and proliferation of human synovium-derived stem cells

•Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate a...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2014-08, Vol.450 (4), p.1593-1599
Main Authors: Kim, Yong Il, Ryu, Jae-Sung, Yeo, Jee Eun, Choi, Yun Jin, Kim, Yong Sang, Ko, Kinarm, Koh, Yong-Gon
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
BIOLOGICAL MARKERS
Cartilage - cytology
Cell Differentiation
CELL PROLIFERATION
Cells, Cultured
Chondrogenic differentiation
COMPARATIVE EVALUATIONS
DNA
Flow Cytometry
GENES
GROWTH FACTORS
HUMAN POPULATIONS
Humans
PHENOTYPE
POLYMERASE CHAIN REACTION
Proliferation
Reverse Transcriptase Polymerase Chain Reaction
STEM CELLS
Stem Cells - cytology
Synovial Membrane - cytology
Synovium-derived mesenchymal stem cell
TGF-β
TOLUIDINE BLUE
TRANSCRIPTION FACTORS
Transduction, Genetic
Transforming Growth Factor beta1 - metabolism
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Summary:•Continuous TGF-β1 overexpression in hSD-MSCs did not influence their phenotypes.•Retroviral-mediated transduction of TGFB1 in hSD-MSCs enhances cell proliferation.•TGF-β1 overexpression did not effect to adipo- or osteogenic potential of hSD-MSCs.•TGF-β1 overexpression in hSD-MSCs could stimulate and accelerate chondrogenesis. Transforming growth factor-beta (TGF-β) superfamily proteins play a critical role in proliferation, differentiation, and other functions of mesenchymal stem cells (MSCs). During chondrogenic differentiation of MSCs, TGF-β up-regulates chondrogenic gene expression by enhancing the expression of the transcription factor SRY (sex-determining region Y)-box9 (Sox9). In this study, we investigated the effect of continuous TGF-β1 overexpression in human synovium-derived MSCs (hSD-MSCs) on immunophenotype, differentiation potential, and proliferation rate. hSD-MSCs were transduced with recombinant retroviruses (rRV) encoding TGF-β1. The results revealed that continuous overexpression of TGF-β1 did not affect their phenotype as evidenced by flow cytometry and reverse transcriptase PCR (RT-PCR). In addition, continuous TGF-β1 overexpression strongly enhanced cell proliferation of hSD-MSCs compared to the control groups. Also, induction of chondrogenesis was more effective in rRV-TGFB-transduced hSD-MSCs as shown by RT-PCR for chondrogenic markers, toluidine blue staining and glycosaminoglycan (GAG)/DNA ratio. Our data suggest that overexpression of TGF-β1 positively enhances the proliferation and chondrogenic potential of hSD-MSCs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2014.07.045