Expression of paired-like homeodomain transcription factor 2c (PITX2c) in epidermal keratinocytes

Paired-like homeodomain transcription factor 2 (PITX2) has been implicated as one of the genes responsible for Rieger syndrome. It has been also shown to play a central role during development. In this study, we investigated the functional role of PITX2 in keratinocyte differentiation. RT–PCR analys...

Full description

Saved in:
Bibliographic Details
Published in:Experimental cell research 2010-11, Vol.316 (19), p.3263-3271
Main Authors: Shi, Ge, Sohn, Kyung-Cheol, Choi, Tae-Young, Choi, Dae-Kyoung, Lee, Sang-Sin, Ou, Bai-sheng, Kim, Sooil, Lee, Young Ho, Yoon, Tae-Jin, Kim, Seong-Jin, Lee, Young, Seo, Young-Joon, Lee, Jeung-Hoon, Kim, Chang Deok
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ADENOVIRUS
Animals
Cell culture
CELL CYCLE
Cell Differentiation
Cell Proliferation
Cellular biology
Danio rerio
Differentiation
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - metabolism
EMBRYOS
Epidermal Cells
EPIDERMIS
Freshwater
GENES
Genetics
GROWTH
Homeobox Protein PITX2
Homeodomain Proteins - metabolism
Humans
IN VIVO
KERATIN
Keratinocytes
Keratinocytes - cytology
Keratinocytes - metabolism
MESSENGER-RNA
MONOCLINIC LATTICES
Paired-like homeodomain transcription factor 2
POLYMERASE CHAIN REACTION
Proliferation
TRANSCRIPTION FACTORS
Transcription Factors - metabolism
Zebrafish
Zebrafish - embryology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Paired-like homeodomain transcription factor 2 (PITX2) has been implicated as one of the genes responsible for Rieger syndrome. It has been also shown to play a central role during development. In this study, we investigated the functional role of PITX2 in keratinocyte differentiation. RT–PCR analysis showed that PITX2c isoform was predominantly expressed in a differentiation-dependent manner. Consistent with, immunohistochemical staining showed that PITX2 expression was increased in the upper layer of epidermis. When PITX2c was overexpressed in cultured keratinocytes by a recombinant adenovirus, the differentiation markers such as involucrin and loricrin were significantly increased at both mRNA and protein levels. In addition, PITX2c overexpression led to the decrease of cell growth, concomitantly with the upregulation of cell cycle-related genes p21. To investigate the effect of PITX2c in vivo, we microinjected PITX2c expression vector into zebrafish embryo. Interestingly, overexpression of PITX2c in zebrafish embryo led to the formation of horn-like structure and thickening of epidermis, together with the increase of keratin 8 (K8) expression. These results suggest that PITX2c has a role in proliferation and differentiation of epidermal keratinocytes.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2010.09.013