Quantum dots-based double-color imaging of HER2 positive breast cancer invasion

► HER2 level is closely related to the biologic behaviors of breast cancer cells. ► A new method to simultaneously image HER2 and type IV collagen was established. ► HER2 status and type IV collagen degradation predict breast cancer invasion. ► The complex interactions between tumor and its environm...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2011-06, Vol.409 (3), p.577-582
Main Authors: Liu, Xiu-Li, Peng, Chun-Wei, Chen, Chuang, Yang, Xue-Qin, Hu, Ming-Bai, Xia, He-Shun, Liu, Shao-Ping, Pang, Dai-Wen, Li, Yan
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
BLOOD VESSELS
Blood Vessels - pathology
Breast cancer invasion
Breast Neoplasms - pathology
COLLAGEN
Collagen Type IV - analysis
Female
Fluorescent Antibody Technique
GROWTH FACTORS
HER2
Humans
MAMMARY GLANDS
METASTASES
Neoplasm Invasiveness
NEOPLASMS
Paraffin Embedding
QUANTUM DOTS
Receptor, ErbB-2 - analysis
Receptor, ErbB-2 - biosynthesis
RECEPTORS
Tissue Embedding
Type IV collagen
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Summary:► HER2 level is closely related to the biologic behaviors of breast cancer cells. ► A new method to simultaneously image HER2 and type IV collagen was established. ► HER2 status and type IV collagen degradation predict breast cancer invasion. ► The complex interactions between tumor and its environment were revealed. It has been well recognized that human epidermal growth factor receptor 2 (HER2) level in breast cancer (BC) is closely related to the malignant biologic behaviors of the tumor, including invasion and metastasis. Yet, there has been a lack of directly observable evidence to support such notion. Here we report a quantum dots (QDs)-based double-color imaging technique to simultaneously show the HER2 level on BC cells and the type IV collagen in the tumor matrix. In benign breast tumor, the type IV collagen was intact. With the increasing of HER2 expression level, there has been a progressive decrease in type IV collagen around the cancer nest. At HER2 (3+) expression level, there has virtually been a total destruction of type IV collagen. Moreover, HER2 (3+) BC cells also show direct invasion into the blood vessels. This novel imaging method provides direct observable evidence to support the theory that the HER2 expression level is directly related to BC invasion.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2011.05.052