The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation

Abstract The P0 protein of poleroviruses and P1 protein of sobemoviruses suppress the plant's RNA silencing machinery. Here we identified a silencing suppressor protein (SSP), P0PE , in the Enamovirus Pea enation mosaic virus -1 (PEMV-1) and showed that it and the P0s of poleroviruses Potato le...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2012-05, Vol.426 (2), p.178-187
Main Authors: Fusaro, Adriana F, Correa, Regis L, Nakasugi, Kenlee, Jackson, Craig, Kawchuk, Lawrence, Vaslin, Maite F.S, Waterhouse, Peter M
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis - virology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
BEANS
CEREALS
Conserved sequence
Enamovirus
Gene Silencing
Infectious Disease
Leaves
Luteoviridae
Luteoviridae - chemistry
Luteoviridae - genetics
Luteoviridae - metabolism
Molecular Sequence Data
Myelin P0 protein
Nucleotide sequence
p1 protein
PEAS
PLANT DISEASES
Plant Diseases - genetics
Plant Diseases - virology
Polerovirus
POTATOES
PROTEINS
Repressor Proteins - chemistry
Repressor Proteins - genetics
Repressor Proteins - metabolism
RNA
RNA Interference
RNA-mediated interference
Sequence Alignment
Silencing suppression
Sobemovirus
Solanum tuberosum
Southern bean mosaic virus
VIRAL DISEASES
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:Abstract The P0 protein of poleroviruses and P1 protein of sobemoviruses suppress the plant's RNA silencing machinery. Here we identified a silencing suppressor protein (SSP), P0PE , in the Enamovirus Pea enation mosaic virus -1 (PEMV-1) and showed that it and the P0s of poleroviruses Potato leaf roll virus and Cereal yellow dwarf virus have strong local and systemic SSP activity, while the P1 of Sobemovirus Southern bean mosaic virus supresses systemic silencing. The nuclear localized P0PE has no discernable sequence conservation with known SSPs, but proved to be a strong suppressor of local silencing and a moderate suppressor of systemic silencing. Like the P0s from poleroviruses, P0PE destabilizes AGO1 and this action is mediated by an F-box-like domain. Therefore, despite the lack of any sequence similarity, the poleroviral and enamoviral SSPs have a conserved mode of action upon the RNA silencing machinery.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2012.01.026