Size-dependent tissue kinetics of PEG-coated gold nanoparticles

Gold nanoparticles (AuNPs) can be used in various biomedical applications, however, very little is known about their size-dependent in vivo kinetics. Here, we performed a kinetic study in mice with different sizes of PEG-coated AuNPs. Small AuNPs (4 or 13 nm) showed high levels in blood for 24 h and...

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Bibliographic Details
Published in:Toxicology and applied pharmacology 2010-05, Vol.245 (1), p.116-123
Main Authors: Cho, Wan-Seob, Cho, Minjung, Jeong, Jinyoung, Choi, Mina, Han, Beom Seok, Shin, Hyung-Seon, Hong, Jin, Chung, Bong Hyun, Jeong, Jayoung, Cho, Myung-Haing
Format: Article
Language:English
Subjects:
ANIMAL TISSUES
Animals
Biological and medical sciences
Blood
BODY
Chemical and industrial products toxicology. Toxic occupational diseases
Cytochrome P-450 CYP1A1 - metabolism
Cytochrome P-450 CYP2B1 - metabolism
DIGESTIVE SYSTEM
ELEMENTS
GLANDS
GOLD
Gold - pharmacokinetics
Gold - urine
Gold nanoparticles
IN VIVO
KINETICS
Kupffer Cells - metabolism
LIVER
Liver - enzymology
Liver - metabolism
Lymph Nodes - metabolism
Lysosomes - metabolism
Male
Medical sciences
Metabolic Detoxication, Phase I
METABOLISM
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
METALS
Metals and various inorganic compounds
Mice
Mice, Inbred BALB C
Mouse
NANOSCIENCE AND NANOTECHNOLOGY
NANOSTRUCTURES
ORGANS
PARTICLE SIZE
Polyethylene Glycols - chemistry
RETICULOENDOTHELIAL SYSTEM
SIZE
Size-dependent kinetics
Spleen - metabolism
Toxicology
TRANSITION ELEMENTS
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Summary:Gold nanoparticles (AuNPs) can be used in various biomedical applications, however, very little is known about their size-dependent in vivo kinetics. Here, we performed a kinetic study in mice with different sizes of PEG-coated AuNPs. Small AuNPs (4 or 13 nm) showed high levels in blood for 24 h and were cleared by 7 days, whereas large (100 nm) AuNPs were completely cleared by 24 h. All AuNPs in blood re-increased at 3 months, which correlated with organ levels. Levels of small AuNPs were peaked at 7 days in the liver and spleen and at 1 month in the mesenteric lymph node, and remained high until 6 months, with slow elimination. In contrast, large AuNPs were taken up rapidly (∼ 30 min) into the liver, spleen, and mesenteric lymph nodes with less elimination phase. TEM showed that AuNPs were entrapped in cytoplasmic vesicles and lysosomes of Kupffer cells and macrophages of spleen and mesenteric lymph node. Small AuNPs transiently activated CYP1A1 and 2B, phase I metabolic enzymes, in liver tissues from 24 h to 7 days, which mirrored with elevated gold levels in the liver. Large AuNPs did not affect the metabolic enzymes. Thus, propensity to accumulate in the reticuloendothelial organs and activation of phase I metabolic enzymes, suggest that extensive further studies are needed for practical in vivo applications.
ISSN:0041-008X
1096-0333
DOI:10.1016/j.taap.2010.02.013