Esco2 is a novel corepressor that associates with various chromatin modifying enzymes

Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid co...

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Published in:	Biochemical and biophysical research communications 2008-07, Vol.372 (2), p.298-304
Main Authors:	Kim, Beom-Jun, Kang, Kyung-Min, Jung, Sung Yun, Choi, Hyun-Kyung, Seo, Jong-Hun, Chae, Ji-Hye, Cho, Eun-Jung, Youn, Hong-Duk, Qin, Jun, Kim, Seong-Tae
Format:	Article
Language:	eng
Subjects:	60 APPLIED LIFE SCIENCES Acetyltransferases - genetics Acetyltransferases - metabolism Cell Line CHROMATIDS CHROMATIN Chromatin - enzymology Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism CHROMOSOMES Cohesion CoREST DNA ENZYMES Esco2 HeLa Cells Histone Methyltransferases Histone-Lysine N-Methyltransferase - genetics Histone-Lysine N-Methyltransferase - metabolism Histones - metabolism Humans LSD1 METHYLATION Methyltransferases - metabolism MITOSIS PROMOTERS Protein Methyltransferases - metabolism Repressor Proteins - genetics Repressor Proteins - metabolism TRANSCRIPTION Transcription, Genetic
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Summary:	Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S phase. Here, we report that Esco2 interacts with several component proteins of the CoREST complex, including a transcription corepressor CoREST, histone demethlyase LSD1, HDAC1, HDAC2, BRAF35, and PHF21A. Esco2 also interacts with various histone methyltransferases Suv39h1, SETDB1 and G9a. Esco2 complex purified from HeLa nuclear extract possesses histone H3 K9 methylation activity and functions as a transcription repressor. Esco2 fused to Gal4 DNA binding domain represses transcription by increasing methylation of histone H3 K9 in the promoter region. These results suggest a novel function of Esco2 in transcription repression through modulation of the chromatin structure.
ISSN:	0006-291X 1090-2104