Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples

Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discriminat...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-05, Vol.356 (4), p.1017-1023
Main Authors: Hsia, Chu Chieh, Chizhikov, Vladimir E., Yang, Amy X., Selvapandiyan, Angamuthu, Hewlett, Indira, Duncan, Robert, Puri, Raj K., Nakhasi, Hira L., Kaplan, Gerardo G.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AIDS VIRUS
BLOOD
Blood-borne virus
Discriminant Analysis
DNA, Viral - blood
GLASS
Hepacivirus - genetics
Hepacivirus - isolation & purification
HEPATITIS
Hepatitis B virus
Hepatitis B virus (HBV)
Hepatitis B virus - genetics
Hepatitis B virus - isolation & purification
Hepatitis C virus
Hepatitis C virus (HCV)
HIV-1 - genetics
HIV-1 - isolation & purification
Human immunodeficiency virus 1
Human immunodeficiency virus type-1 (HIV-1)
Humans
MICROARRAY TECHNOLOGY
Multiplex detection and discrimination
Oligonucleotide Array Sequence Analysis - methods
OLIGONUCLEOTIDES
PATHOGENS
PATIENTS
PCR
POLYMERASE CHAIN REACTION
PUBLIC HEALTH
Reproducibility of Results
Sensitivity and Specificity
TRANSFUSIONS
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Summary:Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.03.087