Characterization and quantification of corticosteroid-binding globulin in a southern toad, Bufo terrestris , exposed to coal-combustion-waste

Abstract Corticosteroid-binding globulin (CBG) is a plasma protein that binds corticosterone and may regulate access of hormone to tissues. The role of CBG during a stress response is not clear. At least two hypotheses have been proposed: 1) CBG levels may increase in response to a stressor, thereby...

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Published in:General and comparative endocrinology 2007-05, Vol.152 (1), p.82-88
Main Authors: Ward, Chelsea K, Fontes, Cristiano, Breuner, Creagh W, Mendonça, Mary T
Format: Article
Language:English
Subjects:
01 COAL, LIGNITE, AND PEAT
Amphibian
AMPHIBIANS
Animals
ASHES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
Bufonidae - blood
COAL
Corticosteroid-binding globulin
Corticosterone
Endocrinology & Metabolism
Environmental Pollutants - pharmacology
Environmental Pollution
FLY ASH
FROGS
GLOBULINS
HORMONES
Industrial Waste
Male
Metal
METALS
Power Plants
Refuse Disposal
STEROIDS
Stress
Time Factors
Transcortin - analysis
WATER POLLUTION
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Summary:Abstract Corticosteroid-binding globulin (CBG) is a plasma protein that binds corticosterone and may regulate access of hormone to tissues. The role of CBG during a stress response is not clear. At least two hypotheses have been proposed: 1) CBG levels may increase in response to a stressor, thereby decreasing the amount of circulating free corticosterone, or 2) CBG levels may decline, making corticosterone available for its role in increased metabolic needs during stress. In this study, southern toads, Bufo terrestris , were exposed to a chronic pollutant (coal-combustion-waste), to determine changes in CBG and free corticosterone levels. Since toads exposed to chronic pollutants in previous studies did not exhibit the predicted changes in metabolic rate and mass, but did experience a significant elevation in total corticosterone, we hypothesized that CBG would likewise increase and thus, mitigate the effects of a chronic (i.e. 2 months) pollutant stressor. To conduct this study, we first characterized the properties of CBG in southern toads. Toad CBG has a Kd = 20.6 ± 1.0 nM and a Bmax = 332.2 ± 5.1 nmol/L plasma. The rank order potencies for steroid inhibition of tritiated corticosterone are: dihydrotestosterone > corticosterone ≫ progesterone = testosterone ⋙ estrogen = dexamethasone. After characterization, we monitored the changes in CBG, total corticosterone, and free corticosterone in male toads that were exposed to either coal-combustion-waste or control conditions. CBG increased in all groups throughout the experiment. Total corticosterone, on the other hand, was only significantly elevated at four weeks of exposure to coal-combustion-waste. The increase in CBG did not parallel the increase in total corticosterone; as a result, free corticosterone levels were not buffered by CBG, but showed a peak at four weeks similar to total corticosterone. This finding indicates that, in this species, CBG may not provide a protective mechanism during long-term pollution exposure.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2007.02.031