The effects of dexamethasone on rat brain cortical nuclear factor kappa B (NF-κB) in endotoxic shock

To explore the molecular mechanism of brain tissue injury induced by lipopolysaccharide (LPS), we studied the effects of endotoxic shock on rat brain cortex NF-κB and the effects of dexamethasone on these changes. Rats were randomly divided into LPS, LPS + dexamethasone, and control groups. The DNA-...

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Bibliographic Details
Published in:Toxicology and applied pharmacology 2006-08, Vol.214 (3), p.263-269
Main Authors: Wang, Zhi, Kang, Jin-song, Li, Yang, Yuan, Zhao-xin, Liu, Shan-shan, Sun, Lian-kun
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Anti-Inflammatory Agents - pharmacology
Biological and medical sciences
Blood Pressure - drug effects
Blotting, Western
BRAIN
Brain injury
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
DEXAMETHASONE
Dexamethasone - pharmacology
DNA
DNA-Binding Proteins - metabolism
Electrophoretic Mobility Shift Assay
Immunohistochemistry
INJURIES
INTRAVENOUS INJECTION
Lipopolysaccharides - toxicity
LPS
Medical sciences
NF-kappa B - antagonists & inhibitors
NF-kappa B - metabolism
NF-κB
Protein Transport - drug effects
PROTEINS
RATS
Rats, Wistar
Shock, Septic - chemically induced
Shock, Septic - metabolism
Shock, Septic - prevention & control
Toxicology
TRANSLOCATION
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Summary:To explore the molecular mechanism of brain tissue injury induced by lipopolysaccharide (LPS), we studied the effects of endotoxic shock on rat brain cortex NF-κB and the effects of dexamethasone on these changes. Rats were randomly divided into LPS, LPS + dexamethasone, and control groups. The DNA-binding activity of NF-κB was observed using electrophoretic mobility shift assay (EMSA). Protein expression in nuclear extracts was studied using Western blots, and nuclear translocation was observed using immunohistochemistry. These indices were assayed at 1 h and 4 h after intravenous injection of LPS (4 mg·kg −1). EMSA showed significantly increased NF-κB DNA-binding activity in nuclear extracts from the LPS group at both 1 h and 4 h after LPS injection, compared with the control group ( P 
ISSN:0041-008X
1096-0333
DOI:10.1016/j.taap.2005.12.014