The PCNA-associated factor KIAA0101/p15(PAF) binds the potential tumor suppressor product p33ING1b

The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using poly...

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Bibliographic Details
Published in:Experimental cell research 2006-01, Vol.312 (1), p.73-85
Main Authors: Simpson, Fiona, Lammerts van Bueren, Kelly, Butterfield, Natalie, Bennetts, Jennifer S, Bowles, Josephine, Adolphe, Christelle, Simms, Lisa A, Young, Joanne, Walsh, Michael D, Leggett, Barbara, Fowles, Lindsay F, Wicking, Carol
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
ANTIGENS
APOPTOSIS
Blotting, Western
Carrier Proteins - genetics
Carrier Proteins - immunology
Carrier Proteins - metabolism
Case-Control Studies
CELL CULTURES
CELL CYCLE
Cell Death - radiation effects
Cell Nucleus - metabolism
CELL PROLIFERATION
Colon - metabolism
Colon - pathology
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
DNA - genetics
DNA - metabolism
DNA REPAIR
DNA REPLICATION
Genes, Tumor Suppressor
HeLa Cells - metabolism
HeLa Cells - radiation effects
Humans
Immunoglobulin G - immunology
Immunoprecipitation
Inhibitor of Growth Protein 1
Intracellular Signaling Peptides and Proteins - metabolism
IRRADIATION
Kidney - metabolism
Kidney - radiation effects
Mice
MITOCHONDRIA
Mutation - genetics
Neoplasm Proteins - genetics
Neoplasm Proteins - immunology
Neoplasm Proteins - metabolism
NEOPLASMS
Nuclear Proteins - metabolism
Proliferating Cell Nuclear Antigen - metabolism
Protein Binding
PROTEINS
RNA - genetics
RNA - metabolism
TUMOR CELLS
Tumor Suppressor Proteins - metabolism
Ultraviolet Rays
YEASTS
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Summary:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera raised against a p15(PAF) fusion protein, we have shown that in a range of mammalian tumor and non-tumor cell lines the endogenous p15(PAF) protein localises to the nucleus and the mitochondria. Under normal conditions no co-localisation with PCNA could be detected, however following exposure to UV it was possible to co-immunoprecipitate p15(PAF) and PCNA from a number of cell lines, suggesting a UV-enhanced association of the two proteins. Overexpression of p15(PAF) in mammalian cells was also found to protect cells from UV-induced cell death. Based on similarities between the behaviour of p15(PAF) and the potential tumor suppressor product p33ING1b, we have further shown that these two proteins interact in the same complex in cell cultures. This suggests that p15(PAF) forms part of a larger protein complex potentially involved in the regulation of DNA repair, apoptosis and cell cycle progression.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2005.09.020