Toxicogenomics concepts and applications to study hepatic effects of food additives and chemicals

Transcriptomics, proteomics and metabolomics are genomics technologies with great potential in toxicological sciences. Toxicogenomics involves the integration of conventional toxicological examinations with gene, protein or metabolite expression profiles. An overview together with selected examples...

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Bibliographic Details
Published in:	Toxicology and applied pharmacology 2005-09, Vol.207 (2), p.179-188
Main Authors:	Stierum, Rob, Heijne, Wilbert, Kienhuis, Anne, van Ommen, Ben, Groten, John
Format:	Article
Language:	eng
Subjects:	60 APPLIED LIFE SCIENCES Animals BIOCHEMISTRY CURCUMIN FOOD ADDITIVES Food Additives - toxicity GENES Genomics Humans IN VITRO IN VIVO LIVER Liver - drug effects PROTEINS RATS TOXICITY Toxicogenomics Toxicology
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Summary:	Transcriptomics, proteomics and metabolomics are genomics technologies with great potential in toxicological sciences. Toxicogenomics involves the integration of conventional toxicological examinations with gene, protein or metabolite expression profiles. An overview together with selected examples of the possibilities of genomics in toxicology is given. The expectations raised by toxicogenomics are earlier and more sensitive detection of toxicity. Furthermore, toxicogenomics will provide a better understanding of the mechanism of toxicity and may facilitate the prediction of toxicity of unknown compounds. Mechanism-based markers of toxicity can be discovered and improved interspecies and in vitro–in vivo extrapolations will drive model developments in toxicology. Toxicological assessment of chemical mixtures will benefit from the new molecular biological tools. In our laboratory, toxicogenomics is predominantly applied for elucidation of mechanisms of action and discovery of novel pathway-supported mechanism-based markers of liver toxicity. In addition, we aim to integrate transcriptome, proteome and metabolome data, supported by bioinformatics to develop a systems biology approach for toxicology. Transcriptomics and proteomics studies on bromobenzene-mediated hepatotoxicity in the rat are discussed. Finally, an example is shown in which gene expression profiling together with conventional biochemistry led to the discovery of novel markers for the hepatic effects of the food additives butylated hydroxytoluene, curcumin, propyl gallate and thiabendazole.
ISSN:	0041-008X 1096-0333