Nuclear actin is partially associated with Cajal bodies in human cells in culture and relocates to the nuclear periphery after infection of cells by adenovirus 5

Cajal bodies are intra-nuclear structures enriched in proteins involved in transcription and mRNA processing. In this study, immunofluorescence microscopy experiments using a highly specific antibody to actin revealed nuclear actin spots that colocalized in part with p80 coilin-positive Cajal bodies...

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Bibliographic Details
Published in:Experimental cell research 2005-02, Vol.303 (2), p.229-239
Main Authors: Gedge, L.J.E., Morrison, E.E., Blair, G.E., Walker, J.H.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ACTIN
Actins - metabolism
ADENOVIRUS
Adenoviruses, Human - pathogenicity
ANIMAL CELLS
ANTIBODIES
Capsid Proteins - metabolism
Cell Line
Cell Nucleus - metabolism
Cell Nucleus - virology
Coiled Bodies - metabolism
Coiled Bodies - virology
HeLa Cells
Humans
MICROSCOPY
Microscopy, Fluorescence
Nuclear matrix
Nuclear Matrix - metabolism
Nuclear Matrix - virology
NUCLEAR STRUCTURE
p80 coilin
TRANSCRIPTION
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Summary:Cajal bodies are intra-nuclear structures enriched in proteins involved in transcription and mRNA processing. In this study, immunofluorescence microscopy experiments using a highly specific antibody to actin revealed nuclear actin spots that colocalized in part with p80 coilin-positive Cajal bodies. Actin remained associated with Cajal bodies in cells extracted to reveal the nuclear matrix. Adenovirus infection, which is known to disassemble Cajal bodies, resulted in loss of actin from these structures late in infection. In infected cells, nuclear actin was observed to relocate to structures at the periphery of the nucleus, inside the nuclear envelope. Based on these findings, it is suggested that actin may play an important role in the organization or function of the Cajal body.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2004.06.034