Enhancement of radiosensitivity in H1299 cancer cells by actin-associated protein cofilin

Cofilin is an actin-associated protein that belongs to the actin depolymerization factor/cofilin family and is important for regulation of actin dynamics. Cofilin can import actin monomers into the nucleus under certain stress conditions, however the biological effects of nuclear transport are uncle...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-09, Vol.335 (2), p.286-291
Main Authors: Lee, Yi-Jang, Sheu, Tzong-Jen, Keng, Peter C.
Format: Article
Language:English
Subjects:
ACTIN
Actin Cytoskeleton - chemistry
Actin Depolymerizing Factors
Actins - chemistry
Actins - metabolism
Active Transport, Cell Nucleus
Antigens, Nuclear - biosynthesis
BIOLOGICAL RADIATION EFFECTS
Blotting, Western
Carcinoma, Non-Small-Cell Lung - metabolism
Cell Line, Tumor
Cell Nucleus - metabolism
Cell Survival
Cofilin
Comet Assay
Comet assays
Contractile Proteins - chemistry
DEPOLYMERIZATION
DNA
DNA Damage
DNA REPAIR
DNA repair capacity
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - metabolism
Dose-Response Relationship, Radiation
Down-Regulation
Humans
IONIZING RADIATIONS
IRRADIATION
Ku Autoantigen
Lung Neoplasms - metabolism
LUNGS
Microfilament Proteins - chemistry
NEOPLASMS
Rad51 Recombinase
Rad52 DNA Repair and Recombination Protein
Radiation Tolerance
Radiation, Ionizing
RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
RADIOSENSITIVITY
STRAND BREAKS
Time Factors
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Summary:Cofilin is an actin-associated protein that belongs to the actin depolymerization factor/cofilin family and is important for regulation of actin dynamics. Cofilin can import actin monomers into the nucleus under certain stress conditions, however the biological effects of nuclear transport are unclear. In this study, we found that over-expression of cofilin led to increased radiation sensitivity in human non-small lung cancer H1299 cells. Cell survival as determined by colony forming assay showed that cells over-expressing cofilin were more sensitive to ionizing radiation (IR) than normal cells. To determine whether the DNA repair capacity was altered in cofilin over-expressing cells, comet assays were performed on irradiated cells. Repair of DNA damage caused by ionizing radiation was detected in cofilin over-expressing cells after 24 h of recovery. Consistent with this observation, the key components for repair of DNA double-strand breaks, including Rad51, Rad52, and Ku70/Ku80, were down-regulated in cofilin over-expressing cells after IR exposure. These findings suggest that cofilin can influence radiosensitivity by altering DNA repair capacity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.07.073