Circulating factors cause proteinuria in parabiotic zebrafish

Proteinuria can be induced by impairment of any component of the glomerular filtration barrier (GFB). To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholin...

Full description

Saved in:
Bibliographic Details
Published in:Kidney international 2019-08, Vol.96 (2), p.342-349
Main Authors: Müller-Deile, J., Schenk, H., Schroder, P., Schulze, K., Bolaños-Palmieri, P., Siegerist, F., Endlich, N., Haller, H., Schiffer, M.
Format: Article
Language:English
Subjects:
nephrotic syndrome
podocyte
proteinuria
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Proteinuria can be induced by impairment of any component of the glomerular filtration barrier (GFB). To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholino-mediated knockdown of a podocyte specific gene (nephronectin) was induced in one zebrafish larva which was then fused to an un-manipulated fish. Notably, proteinuria and glomerular damage were present in the manipulated fish and in the parabiotically-fused partner. Thus, circulating permeability factors may be induced by proteinuria even when an induced podocyte gene dysregulation is the initiating cause. [Display omitted]
ISSN:0085-2538
1523-1755
DOI:10.1016/j.kint.2019.02.013