Long-lived and short-lived reactive species produced by a cold atmospheric pressure plasma jet for the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus

Different chemical pathways leading to the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus by a cold atmospheric pressure plasma jet (APPJ) in buffered and non-buffered solutions are reported. As APPJs produce a complex mixture of reactive species in solution, a comprehensive set of...

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Bibliographic Details
Published in:Free radical biology & medicine 2018-08, Vol.124 (C), p.275-287
Main Authors: Kondeti, V.S. Santosh K., Phan, Chi Q., Wende, Kristian, Jablonowski, Helena, Gangal, Urvashi, Granick, Jennifer L., Hunter, Ryan C., Bruggeman, Peter J.
Format: Article
Language:English
Subjects:
Atmospheric pressure plasma
Bacteria inactivation
Biochemistry & Molecular Biology
ClO
Electron spin resonance
Endocrinology & Metabolism
O2
OH
Plasma medicine
Pseudomonas aeruginosa
Staphylococcus aureus
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Summary:Different chemical pathways leading to the inactivation of Pseudomonas aeruginosa and Staphylococcus aureus by a cold atmospheric pressure plasma jet (APPJ) in buffered and non-buffered solutions are reported. As APPJs produce a complex mixture of reactive species in solution, a comprehensive set of diagnostics were used to assess the liquid phase chemistry. This includes absorption and electron paramagnetic resonance spectroscopy in addition to a scavenger study to assess the relative importance of the various plasma produced species involved in the inactivation of bacteria. Different modes of inactivation of bacteria were found for the same plasma source depending on the solution and the plasma feed gas. The inactivation of bacteria in saline is due to the production of short-lived species in the case of argon plasma when the plasma touches the liquid. Long-lived species (ClO−) formed by the abundant amount of O. radicals produced by the plasmas played a dominant role in the case of Ar + 1% O2 and Ar + 1% air plasmas when the plasma is not in direct contact with the liquid. Inactivation of bacteria in distilled water was found to be due to the generation of short-lived species: O. &O2.− for Ar + 1% O2 plasma and O2.− (and .OH in absence of saline) for Ar plasma. [Display omitted] •Plasma-induced inactivation of P. aeruginosa can involve different reactive species.•In the presence of saline, Ar-O2 and Ar-air plasma leads to the formation of ClO−.•In saline, the ClO− concentration correlates with bacteria inactivation.•Argon plasma in direct contact with the liquid leads to short-lived reactive species.•The key short-lived species involved in the inactivation are ·OH and superoxide.•The role of .OH radicals is suppressed in 0.9% saline solution.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2018.05.083