Bacterial analysis by MALDI-TOF mass spectrometry: An inter-laboratory comparison

Bacterial analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated in numerous laboratories, and a few attempts have been made to compare results from different laboratories on the same organism. It has been difficult to understand th...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2005-04, Vol.16 (4), p.456-462
Main Authors: Wunschel, Sharon C., Jarman, Kristin H., Petersen, Catherine E., Valentine, Nancy B., Wahl, Karen L., Schauki, Dunja, Jackman, Joany, Nelson, Chad P., White, Edward
Format: Article
Language:English
Subjects:
Automation
BACTERIA
Bacterial Proteins - analysis
Bacteriology
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
Data processing
Desorption
Escherichia coli - chemistry
Escherichia coli - metabolism
Fingerprints
Fundamental and applied biological sciences. Psychology
General aspects
Identification methods
INTERLABORATORY COMPARISONS
Ionization
Ions
Laboratories
Lasers
MASS SPECTRA
Mass spectrometry
Microbiology
PATTERN RECOGNITION
Peptide Mapping - methods
Proteome
Reproducibility
Reproducibility of Results
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Spectroscopy
TIME-OF-FLIGHT MASS SPECTROMETERS
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Description
Summary:Bacterial analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated in numerous laboratories, and a few attempts have been made to compare results from different laboratories on the same organism. It has been difficult to understand the causes behind the observed differences between laboratories when different instruments, matrices, solvents, etc. are used. In order to establish this technique as a useful tool for bacterial identification, additional efforts in standardizing the methods by which MALDI mass spectra are obtained and comparisons of spectra from different instruments with different operators are needed. Presented here is an extension of our previous single-laboratory reproducibility study with three different laboratories in a controlled experiment with aliquots of the same bacterial culture, matrix stock solution, and calibrant standards. Using automated spectral collection of whole-cell bacteria and automated data processing and analysis algorithms, fingerprints from three different laboratories were constructed and compared. Nine of the ions appeared reproducibly within all three laboratories, with additional unique ions observed within each of the laboratories. An initial evaluation of the ability to use a fingerprint generated within one laboratory for bacterial identification of a sample from another laboratory is presented, and strategies for improving identification rates between laboratories is discussed.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2004.12.004