Multiple-targeted graphene-based nanocarrier for intracellular imaging of mRNAs

Simultaneous detection and imaging of multiple intracellular messenger RNA (mRNAs) hold great significant for early cancer diagnostics and preventive medicine development. Herein, we propose a multiple-targeted graphene oxide (GO) nanocarrier that can simultaneously detect and image different type m...

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Bibliographic Details
Published in:Analytica chimica acta 2017-08, Vol.983 (2017), p.1-8
Main Authors: Wang, Ying, Li, Zhaohui, Liu, Misha, Xu, Jinjin, Hu, Dehong, Lin, Yuehe, Li, Jinghong
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Actin
BASIC BIOLOGICAL SCIENCES
Biocompatibility
Cancer
Cell Line, Tumor
Cells (biology)
Deoxyribonucleic acid
DNA
DNA Probes
DNA, Single-Stranded
Environmental Molecular Sciences Laboratory
Fluorescence
Fluorescent Dyes
Fluorescent indicators
Gene expression
Graphene
Graphene oxide
Graphite
Humans
Image detection
Imaging
Intracellular
Living cell
Manganese
MicroRNAs
mRNA
Multiple detection
Nanoparticles
Nuclease
Oxides
Preventive medicine
Probes
Ribonucleic acid
RNA
RNA, Messenger - analysis
Studies
Superoxide dismutase
Target detection
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Summary:Simultaneous detection and imaging of multiple intracellular messenger RNA (mRNAs) hold great significant for early cancer diagnostics and preventive medicine development. Herein, we propose a multiple-targeted graphene oxide (GO) nanocarrier that can simultaneously detect and image different type mRNAs in living cells. First of all, in vitro detection of multiple targets have been realized successfully based on the multiple-targeted GO nanocarrier with linear relationship ranging from 3 nM to 200 nM, as well as sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA. Additionally, this nanosensing platform composed of fluorescent labelled single strand DNA probes and GO nanocarrier can identify Mn-SOD mRNA and endogenous mRNA of β-actin in living cancer cells, showing rapid response, high specificity, nuclease stability, and good biocompatibility during the cell imaging. Thirdly, changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully. By using multiple ssDNA as probes and GO nanocarrier as the cellular delivery cargo, the proposed simultaneous multiple-targeted sensing platform will be of great potential as a powerful tool for intracellular trafficking process from basic research to clinical diagnosis. Schematic illustration of simultaneously multiple mRNAs monitoring inside single living breast cancer cell based on GO nanocarrier. In particular, the fluorescent signals could be monitored when Mn-SOD probe (red) and β-actin probe (green) hybridizes with their mRNA targets inside the living cells. Random probe (orange) was regarded as control probe for the sensing strategy. [Display omitted] •A multiple-targeted GO nanocarrier was used for mRNAs imaging and expression changes after drug treatment can be monitored successfully.•Sensitive detection limit of 1.84 nM for manganese superoxide dismutase (Mn-SOD) mRNA and 2.45 nM for β-actin mRNA was accomplished.•Changes of the expression levels of mRNA in living cells before or after the drug treatment can be monitored successfully.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2017.07.017