High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System

Let enzymes work: H2 was produced from xylose and water in one reactor containing 13 enzymes (red). By using a novel polyphosphate xylulokinase (XK), xylose was converted into H2 and CO2 with approaching 100 % of the theoretical yield. The findings suggest that cell‐free biosystems could produce H2...

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Bibliographic Details
Published in:Angewandte Chemie (International ed.) 2013-04, Vol.52 (17), p.4587-4590
Main Authors: Martín del Campo, Julia S., Rollin, Joseph, Myung, Suwan, Chun, You, Chandrayan, Sanjeev, Patiño, Rodrigo, Adams, Michael WW, Zhang, Y.-H. Percival
Format: Article
Language:English
Subjects:
Acid Anhydride Hydrolases - chemistry
Aldose-Ketose Isomerases - chemistry
Biocatalysis
Biomass
Carbon dioxide
Cascades
Cell-Free System - chemistry
Cell-Free System - enzymology
Enzymes
Enzymes, Immobilized - chemistry
hydrogen
Hydrogen - chemistry
Hydrogenase - chemistry
hydrogenases
Low cost
Polyphosphates
Reactors
Xylose
Xylose - chemistry
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Summary:Let enzymes work: H2 was produced from xylose and water in one reactor containing 13 enzymes (red). By using a novel polyphosphate xylulokinase (XK), xylose was converted into H2 and CO2 with approaching 100 % of the theoretical yield. The findings suggest that cell‐free biosystems could produce H2 from biomass xylose at low cost. Xu5P=xylulose 5‐phosphate, G6P=glucose 6‐phosphate.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201300766