Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s

Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s⁻¹. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than ∼1 s. However, at T = 260 K...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2012-02, Vol.68 (Pt 2), p.124-133
Main Authors: Warkentin, Matthew, Badeau, Ryan, Hopkins, Jesse B, Mulichak, Anne M, Keefe, Lisa J, Thorne, Robert E
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
Crystallization
CRYSTALLOGRAPHY
Crystallography, X-Ray
DOSE RATES
GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
Plant Proteins - chemistry
Plants - chemistry
PROTEINS
Radiation Dosage
RADIATIONS
SENSITIVITY
X-Rays
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Summary:Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s⁻¹. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than ∼1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of ∼10 kGy s⁻¹ can be outrun by collecting data at 680 kGy s⁻¹. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.
ISSN:0907-4449
1399-0047
DOI:10.1107/S0907444911052085