Phase Preference by Active, Acetate-Utilizing Bacteria at the Rifle, CO Integrated Field Research Challenge Site

Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used...

Full description

Saved in:

Bibliographic Details
Published in:	Environmental science & technology 2011-02, Vol.45 (4), p.1250-1256
Main Authors:	Kerkhof, Lee J, Williams, Ken H, Long, Philip E, McGuinness, Lora R
Format:	Article
Language:	eng
Subjects:	ACETATES Acetates - metabolism active bacteria AMPLIFICATION Applied sciences BACTERIA Bacteria - isolation & purification Bacteria - metabolism BASIC BIOLOGICAL SCIENCES Biodegradation, Environmental Biological and medical sciences Biological treatment of waters Biotechnology Characterization of Natural and Affected Environments Chemical compounds Chemical synthesis Colorado CONTAMINATION Decontamination. Miscellaneous Deoxyribonucleic acid DNA Earth sciences Earth, ocean, space Engineering and environment geology. Geothermics Environment and pollution Environmental science ENVIRONMENTAL SCIENCES Exact sciences and technology Fundamental and applied biological sciences. Psychology GROUND WATER Groundwater Groundwater - microbiology Groundwaters Industrial applications and implications. Economical aspects Isotopes MICROORGANISMS Natural water pollution Pollution Pollution, environment geology RADIOISOTOPES SAND sediment-associated microbes, Rifle IFRC SIP Soil and sediments pollution Soil Microbiology Soil Pollutants, Radioactive - metabolism URANIUM uranium reduction Water treatment and pollution
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Previous experiments at the Rifle, Colorado Integrated Field Research Challenge (IFRC) site demonstrated that field-scale addition of acetate to groundwater reduced the ambient soluble uranium concentration. In this report, sediment samples collected before and after acetate field addition were used to assess the active microbes via 13C acetate stable isotope probing on 3 phases [coarse sand, fines (8-approximately 150 μm), groundwater (0.2−8 μm)] over a 24-day time frame. TRFLP results generally indicated a stronger signal in 13C-DNA in the “fines” fraction compared to the sand and groundwater. Before the field-scale acetate addition, a Geobacter-like group primarily synthesized 13C-DNA in the groundwater phase, an alpha Proteobacterium primarily grew on the fines/sands, and an Acinetobacter sp. and Decholoromonas-like OTU utilized much of the 13C acetate in both groundwater and particle-associated phases. At the termination of the field-scale acetate addition, the Geobacter-like species was active on the solid phases rather than the groundwater, while the other bacterial groups had very reduced newly synthesized DNA signal. These findings will help to delineate the acetate utilization patterns of bacteria in the field and can lead to improved methods for stimulating distinct microbial populations in situ.
ISSN:	0013-936X 1520-5851