Modulation of the neurological and vascular complications by grape seed extract in a rat model of spinal cord ischemia–reperfusion injury by downregulation of both osteopontin and cyclooxygenase-2

In this study, we investigated the effects of grape seed extract (GSE) on the expression of osteopontin (OPN) and cyclooxygenase-2 (COX-2) in a rat model of spinal cord ischemia–reperfusion injury (SC-IRI). Fifty male rats were divided into 5 groups: control (CON); control + GSE (CON + GSE) (receive...

Full description

Saved in:
Bibliographic Details
Published in:Canadian journal of physiology and pharmacology 2016-07, Vol.94 (7), p.719-727
Main Authors: Sakr, Hussein F, Abbas, Amr M, Bin-Jaliah, Ismaeel
Format: Article
Language:English
Subjects:
Animals
Blood circulation disorders
Care and treatment
Central nervous system diseases
Complications and side effects
Cyclooxygenase 2 - metabolism
cyclooxygenase-2
Cyclooxygenases
Disease Models, Animal
Down-Regulation - drug effects
Down-Regulation - physiology
extrait de pépins de raisin
Fruits
Glycoproteins
grape seed extract
Grape Seed Extract - pharmacology
Grape Seed Extract - therapeutic use
Health aspects
Ischemia
ischemia–reperfusion injury
lésion d’ischémie–reperfusion
Male
Nervous system diseases
Neuroprotective Agents - pharmacology
Neuroprotective Agents - therapeutic use
osteopontin
Osteopontin - antagonists & inhibitors
Osteopontin - metabolism
ostéopontine
oxidative stress
Oxidative Stress - drug effects
Oxidative Stress - physiology
Prevention
Proteins
Random Allocation
Rats
Rats, Sprague-Dawley
Reperfusion injury
Reperfusion Injury - drug therapy
Reperfusion Injury - metabolism
Reperfusion Injury - pathology
Risk factors
Rodents
Seeds
Spinal Cord - drug effects
Spinal Cord - metabolism
Spinal Cord - pathology
stress oxydatif
Vitaceae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we investigated the effects of grape seed extract (GSE) on the expression of osteopontin (OPN) and cyclooxygenase-2 (COX-2) in a rat model of spinal cord ischemia–reperfusion injury (SC-IRI). Fifty male rats were divided into 5 groups: control (CON); control + GSE (CON + GSE) (received GSE for 28 days); sham operated (Sham); IRI; and IRI + GSE. SC-IRI was induced by clamping the aorta just above the bifurcation for 45 min, and then the clamp was released for 48 h for reperfusion. IRI + GSE group received GSE for 28 days before SC-IRI. Sensory, motor, and placing/stepping reflex assessment was performed. Prostaglandin E2 (PGE2), thiobarbituric acid reactive substances (TBARs), and total antioxidant capacity (TAC) were measured in spinal cord homogenate. Immunohistochemical examination of the spinal cord for OPN and COX-2 were carried out. SC-IRI resulted in significant increase in plasma nitrite/nitrate level and spinal cord homogenate levels of TBARs and PGE2, and OPN and COX-2 expression with significant decrease in TAC. GSE improves the sensory and motor functions through decreasing OPN and COX-2 expression with reduction of oxidative stress parameters. We conclude a neuroprotective effect of GSE in SC-IRI through downregulating COX-2 and OPN expression plus its antioxidants effects.
ISSN:0008-4212
1205-7541
DOI:10.1139/cjpp-2015-0498