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Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five dif...

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Bibliographic Details
Published in:BMB reports 2008-09, Vol.41 (9), p.670-677
Main Authors: Pongsomboon, Siriporn, Tang, Sureerat, Boonda, Suleeporn, Aoki, Takashi, Hirono, Ikuo, Yasuike, Motoshige, Tassanakajon, Anchalee
Format: Article
Language:Korean
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Summary:A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly ($\leq$ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.
ISSN:1976-6696
1976-670X