The Leukocyte Antigen CD43 Is Expressed in Different Cell Lines of Nonhematopoietic Origin

CD43 is an abundant transmembrane sialoglycoprotein in leukocyte-type cell lines, but it has also been suggested to be present in colon adenomas and colon carcinomas. We have now shown that CD43 is expressed in a variety of cell lines of different origins (CaSKI, A549, 293, MTSV1-7, MCF7, HT-1080, J...

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Bibliographic Details
Published in:Tumor biology 2002-07, Vol.23 (4), p.193-201
Main Authors: Fernandez-Rodriguez, Julia, Andersson, Christian X., Laos, Sirle, Baeckström, Dan, Sikut, Anu, Sikut, Rein, Hansson, Gunnar C.
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal - metabolism
Antigens
Antigens, CD
Biological and medical sciences
biosynthesis
Blotting
Blotting, Western
CD43
Cell Line
Cell Separation
Confocal
Cultured
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Exons
Flow Cytometry
Fluorescence
Host-tumor relations. Immunology. Biological markers
Humans
Introns
Jurkat Cells
Leukosialin
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Medical sciences
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
metabolism
Microscopy
Microscopy, Confocal
Microscopy, Fluorescence
Monoclonal
Plasmids
Plasmids - metabolism
Polyacrylamide Gel
Precipitin Tests
Research Article
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins
Sialoglycoproteins - biosynthesis
Sialoglycoproteins - metabolism
Tumor Cells
Tumor Cells, Cultured
Tumors
Western
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Description
Summary:CD43 is an abundant transmembrane sialoglycoprotein in leukocyte-type cell lines, but it has also been suggested to be present in colon adenomas and colon carcinomas. We have now shown that CD43 is expressed in a variety of cell lines of different origins (CaSKI, A549, 293, MTSV1-7, MCF7, HT-1080, Jurkat, K562, COLO 205, HT-29, Caco-2, DLD-1 and SW480). The level of expression of CD43 mRNA was analyzed by reverse transcriptase-polymerase chain reaction and that of the protein by immunoprecipitation and Western blot, flow cytometry and confocal microscopy using two monoclonal anti-CD43 antibodies (L10 and 4D2). As all cell lines expressed CD43, it is suggested that CD43 has a more fundamental function than previously believed and thus cannot be considered only as a specific leukocyte marker.
ISSN:1010-4283
1423-0380
DOI:10.1159/000067252